Genetically transformed Bifidobacterium longum, a probiotic bacterium in the human intestines, has a high potential for in-situ delivery of therapeutic proteins. However, one limitation is its low transformation efficiency caused by its thick membrane as it is a Gram-positive bacterium. Here we present an excellent transformation efficiency of B. longum (up to 1 × 10⁷ to 2.1 × 10⁷ cells/μg of DNA), obtained when oxidized multi-walled carbon nanotubes are used in synergy with classic transformation methods. This approach opens a fruitful avenue for B. longum transformations that could increase its potential for in-situ delivery systems of recombinant proteins.

经基因改造的长双歧杆菌是人类肠道中的一种益生菌，在原位递送治疗性蛋白质方面具有很高的潜力。然而，一个限制是由于其厚膜导致其转化效率低，因为它是一种革兰氏阳性细菌。在这里，我们展示了长双歧杆菌的出色转化效率（高达 1 × 10 ⁷至 2.1 × 10 ^{7 个} 细胞/μg DNA），当氧化多壁碳纳米管与经典转化方法协同使用时获得。这种方法为B. longum转化开辟了一条富有成效的途径，可以增加其原位转化的潜力重组蛋白的传递系统。