Objectives

MicroRNA-199b-3p (miR-199b-3p) plays a crucial role in the malignant development of various cancers, but little known in prostate cancer (PCa). The aim of our study was to demonstrate the function of miR-199b-3p in PCa.

Methods

Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect miR-199b-3p expression in PCa and benign prostatic hyperplasia (BPH) tissue samples. In addition, we examined the relationship between the poor prognosis in PCa and miR-199b-3p. Western blot was used to analyze the expression of Phospholipase Cε (PLCε). CCK8 and colony-forming assays were applied to detect the proliferation of PCa. EdU assay is used to detect PCa cells uptake of EdU. Luciferase reporter assay was applied to analyze the binding between miR-199b-3p and PLCε.

Results

It has been shown that miR-199b-3p in PCa was significantly lower than that in benign prostatic hyperplasia and correlated with poor prognosis. Meanwhile, upregulation of miR-199b-3p can prominently inhibit the proliferation of PCa cells, while its down-regulation triggered opposite result. PLCε was identified as the downstream binding target gene and negatively associated with that of miR-199b-3p.

Conclusion

miR-199b-3p suppresses malignant proliferation by inhibiting PLCε in prostate cancer in vitro and vivo.

中文翻译：

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MicroRNA-199b-3p 通过靶向磷脂酶 Cε 抑制恶性增殖，并与前列腺癌的不良预后相关

目标

MicroRNA-199b-3p (miR-199b-3p) 在各种癌症的恶性发展中起着至关重要的作用，但在前列腺癌 (PCa) 中鲜为人知。我们研究的目的是证明 miR-199b-3p 在 PCa 中的功能。

方法

定量实时聚合酶链反应 (RT-qPCR) 用于检测PCa 和良性前列腺增生 (BPH) 组织样本中的miR-199 b -3p 表达。此外，我们检查了 PCa 和 miR-199b-3p 预后不良之间的关系。使用蛋白质印迹分析磷脂酶 Cε (PLCε) 的表达。应用 CCK8 和集落形成试验来检测 PCa 的增殖。EdU 检测用于检测 PCa 细胞对 EdU 的吸收。应用荧光素酶报告基因分析来分析 miR-199b-3p 与 PLCε 之间的结合。

结果

已经表明，PCa 中的 miR-199b-3p 显着低于良性前列腺增生中的 miR-199b-3p，并且与预后不良相关。同时，miR-199b-3p的上调可以显着抑制PCa细胞的增殖，而其下调引发相反的结果。PLCε 被鉴定为下游结合靶基因，与 miR-199b-3p 的结合靶基因呈负相关。

结论

miR-199b-3p 通过在体外和体内抑制前列腺癌中的 PLCε 来抑制恶性增殖。