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Effect of pesticides on erythrocytes of indigenous fish Labeo rohita
Journal of King Saud University-Science ( IF 3.8 ) Pub Date : 2021-08-28 , DOI: 10.1016/j.jksus.2021.101586
Naheed Bano 1 , Amir Nadeem 2 , Sadia Maalik 3 , Sajida Mushtaq 3 , Naeem Iqbal 4 , Ahmad Kamran Khan 5 , Asghar Shabbir 6 , Muhammad Imran 7 , Safia Niaz 8 , Muhammad Yasin 9 , Mirza Abdul Qayyum 4 , Bashir Ahmad 10 , Milan Skalicky 11 , Sulaiman Ali Alharbi 12 , Saleh Alfarraj 13
Affiliation  

Single cell gel electrophoresis was used to observe the effect of bifenthrin and chlorpyrifos on Labeo rohita. The present work was planned to study the genotoxic effect of pesticide mixture on the major indian carp, L. rohita at sub-lethal concentration 33% LC50. First of all, 96-hr LC50 was determined for L. rohita, then fish was given sub-lethal environment for 70 days. Erythrocytes from blood were taken after each fortnight for the assessment of time dependent DNA damage (% damage), aggregate tail length of comets and genomic destruction. There was increase in damage up to 56 days and after that a slight decrease was observed in next fourteen days. After statistical analysis, it was concluded that the Single Cell Gel Electrophoresis may be implemented in ecofriendly observer courses in evaluation of toxicity.



中文翻译:

农药对本地鱼Labeo rohita红细胞的影响

采用单细胞凝胶电泳观察联苯菊酯和毒死蜱对Labeo rohita的影响。目前的工作计划研究农药混合物在亚致死浓度 33% LC 50 下对主要印度鲤鱼L. rohita的遗传毒性作用。首先,确定了L. rohita 的96 小时 LC 50,然后鱼被给予亚致死环境 70 天。每两周后从血液中取出红细胞用于评估时间依赖性 DNA 损伤(损伤百分比)、彗星的总尾长和基因组破坏。损害增加到 56 天,此后在接下来的 14 天内观察到轻微减少。经过统计分析,得出结论,单细胞凝胶电泳可以在生态友好的观察员课程中进行毒性评估。

更新日期:2021-09-12
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