The purpose of this pilot study was to investigate the effects of the transfusion of one erythrocyte concentrate on the number of circulating red blood cell extracellular vesicles (RBC-EVs) and their clearance time. Six, healthy volunteers donated their blood and were transfused with their RBC concentrate after 35–36 days of storage. One K₂EDTA and one serum sample were collected before donation, at four timepoints after donation and at another six timepoints after transfusion. RBC-EVs were analyzed on a Cytek Aurora flow cytometer. A highly significant increase (p < 0.001) of RBC-EVs from an average of 60.1 ± 19.8 (10³/μL) at baseline to 179.3 ± 84.7 (10³/μL) in the first 1–3 h after transfusion could be observed. Individual differences in the response to transfusion became apparent with one volunteer showing no increase and another an increased concentration at one timepoint after donation due to an influenza infection. We concluded that in an individualized passport approach, increased RBC-EVs might be considered as additional evidence when interpreting suspicious Athletes Biological Passport (ABPs) but for this additional research related to sample collection and transport processes as well as method development and harmonization would be necessary.

中文翻译：

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自体血液掺杂过程中红细胞来源的细胞外囊泡

这项初步研究的目的是研究输注一种红细胞浓缩液对循环红细胞胞外囊泡 (RBC-EV) 数量及其清除时间的影响。六名健康志愿者献血，并在储存 35-36 天后输注红细胞浓缩物。在捐赠前、捐赠后的四个时间点和输血后的另外六个时间点收集了一份K _{2 EDTA 和一份血清样本。}在 Cytek Aurora 流式细胞仪上分析 RBC-EV。 RBC-EVs 从基线时的平均 60.1 ± 19.8 (10 ³ /μL)显着增加 ( p < 0.001)至 179.3 ± 84.7 (10 ³/μL) 在输血后的前 1-3 小时内可以观察到。对输血反应的个体差异变得明显，一名志愿者由于流感感染在献血后的某个时间点浓度没有增加，而另一名志愿者浓度增加。我们的结论是，在个性化护照方法中，在解释可疑运动员生物护照 (ABP) 时，增加的 RBC-EV 可能被视为额外证据，但对于这种与样本收集和运输过程以及方法开发和协调相关的额外研究是必要的.