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Comparison of Serum, Plasma, and Liver Zinc Measurements by AAS, ICP-OES, and ICP-MS in Diverse Laboratory Settings
Biological Trace Element Research ( IF 3.9 ) Pub Date : 2021-08-28 , DOI: 10.1007/s12011-021-02883-z
Andrew G Hall 1, 2 , Janet C King 1, 2 , Christine M McDonald 1, 3
Affiliation  

Progress improving zinc nutrition globally is slowed by limited understanding of population zinc status. This challenge is compounded when small differences in measurement can bias the determination of zinc deficiency rates. Our objective was to evaluate zinc analytical accuracy and precision among different instrument types and sample matrices using a standardized method. Participating laboratories analyzed zinc content of plasma, serum, liver samples, and controls, using a standardized method based on current practice. Instrument calibration and drift were evaluated using a zinc standard. Accuracy was evaluated by percent error vs. reference, and precision by coefficient of variation (CV). Seven laboratories in 4 countries running 9 instruments completed the exercise: 4 atomic absorbance spectrometers (AAS), 1 inductively coupled plasma optical emission spectrometer (ICP-OES), and 4 ICP mass spectrometers (ICP-MS). Calibration differed between individual instruments up to 18.9% (p < 0.001). Geometric mean (95% CI) percent error was 3.5% (2.3%, 5.2%) and CV was 2.1% (1.7%, 2.5%) overall. There were no significant differences in percent error or CV among instrument types (p = 0.91, p = 0.15, respectively). Among sample matrices, serum and plasma zinc measures had the highest CV: 4.8% (3.0%, 7.7%) and 3.9% (2.9%, 5.4%), respectively (p < 0.05). When using standardized materials and methods, similar zinc concentration values, accuracy, and precision were achieved using AAS, ICP-OES, or ICP-MS. However, method development is needed for improvement in serum and plasma zinc measurement precision. Differences in calibration among instruments demonstrate a need for harmonization among laboratories.



中文翻译:

在不同实验室环境中通过 AAS、ICP-OES 和 ICP-MS 测量血清、血浆和肝锌的比较

由于对人口锌状况的了解有限,全球改善锌营养的进展放缓。当测量中的微小差异会影响锌缺乏率的确定时,这一挑战变得更加复杂。我们的目标是使用标准化方法评估不同仪器类型和样品基质之间的锌分析准确度和精密度。参与的实验室使用基于当前实践的标准化方法分析了血浆、血清、肝脏样本和对照的锌含量。使用锌标准评估仪器校准和漂移。准确度通过误差百分比与参考值进行评估,准确度通过变异系数 (CV) 进行评估。4 个国家的 7 个实验室运行 9 台仪器完成了这项工作:4 台原子吸收光谱仪 (AAS),1 台电感耦合等离子体发射光谱仪 (ICP-OES) 和 4 台 ICP 质谱仪 (ICP-MS)。各个仪器之间的校准差异高达 18.9% (p  < 0.001)。几何平均 (95% CI) 百分比误差为 3.5% (2.3%, 5.2%),总体 CV 为 2.1% (1.7%, 2.5%)。仪器类型之间的百分比误差或 CV 没有显着差异(分别为p  = 0.91,p = 0.15)。在样品基质中,血清和血浆锌的 CV 值最高:分别为 4.8%(3.0%、7.7%)和 3.9%(2.9%、5.4%)(p  < 0.05)。使用标准化材料和方法时,使用 AAS、ICP-OES 或 ICP-MS 可实现相似的锌浓度值、准确度和精密度。然而,需要开发方法来提高血清和血浆锌的测量精度。仪器之间校准的差异表明实验室之间需要协调一致。

更新日期:2021-08-28
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