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Characterization of extracellular vesicle miRNA identified in peripheral blood of chronic pancreatitis patients.
Molecular and Cellular Biochemistry ( IF 4.3 ) Pub Date : 2021-08-27 , DOI: 10.1007/s11010-021-04248-5
Chirag S Desai 1 , Aisha Khan 2 , Michael A Bellio 2 , Micah L Willis 3, 4 , Cressida Mahung 3 , Xiaobo Ma 1 , Xavier Baldwin 1 , Brittney M Williams 1 , Todd H Baron 5 , Leon G Coleman 6 , Shannon M Wallet 7, 8 , Robert Maile 3, 4, 8
Affiliation  

Plasma-derived extracellular vesicles (EV) can serve as markers of cell damage/disease but can also have therapeutic utility depending on the nature of their cargo, such as miRNA. Currently, there are challenges and lack of innovations regarding early diagnosis and therapeutic options within different aspects of management of patients suffering from chronic pancreatitis (CP). Use of EV as biomarkers for pancreatic health and/or as adjuvant therapy would make a difference in management of these patients. The aim of this study was to characterize the miRNA cargo of EV purified from the plasma of CP patients and compared to those of healthy participants. EVs were isolated from plasma of 15 CP patients and 10 healthy controls. Nanoparticle tracking analysis was used to determine frequency and size, while NanoString technology was used to characterize the miRNA cargo. Relevant clinical parameters were correlated with EV miRNA cargo. ~ 30 miRNA species were identified to have significantly (p < 0.05) different expression in EV from individuals with CP compared to healthy individuals; ~ 40 miRNA were differentially expressed in EV from pre-diabetic versus non-diabetic CP patients. miR-579-3p, while exhibiting significantly lower (~ 16-fold) expression in CP compared to healthy and lower (~ 24-fold) in CP narcotic users compared to the non-users, is actually enriched (~ 32-fold) within EV in pre-diabetic CP patients compared to non-diabetic CP patients. A unique pattern was identified in female CP patients. These data support the prospect of using a plasma-derived EV cargo to assess pancreatic health and its therapeutic potential in CP patients.

中文翻译:

慢性胰腺炎患者外周血中鉴定的细胞外囊泡 miRNA 的特征。

血浆来源的细胞外囊泡 (EV) 可以作为细胞损伤/疾病的标志物,但也可以根据其货物的性质(例如 miRNA)具有治疗效用。目前,慢性胰腺炎(CP)患者管理的不同方面在早期诊断和治疗选择方面存在挑战和缺乏创新。使用 EV 作为胰腺健康的生物标志物和/或作为辅助治疗将对这些患者的管理产生影响。本研究的目的是表征从 CP 患者血浆中纯化的 EV 的 miRNA 货物,并与健康参与者进行比较。从 15 名 CP 患者和 10 名健康对照者的血浆中分离出 EV。纳米粒子跟踪分析用于确定频率和大小,而 NanoString 技术用于表征 miRNA 货物。相关临床参数与 EV miRNA 货物相关。~ 30 种 miRNA 被鉴定为在 CP 患者的 EV 中与健康个体相比具有显着(p < 0.05)不同的表达;~ 40 种 miRNA 在糖尿病前期与非糖尿病 CP 患者的 EV 中差异表达。miR-579-3p,虽然在 CP 中与健康相比表现出显着较低(~ 16 倍)的表达,并且与非使用者相比在 CP 麻醉剂使用者中表现出较低(~ 24 倍),但实际上是丰富的(~ 32 倍)与非糖尿病 CP 患者相比,糖尿病前期 CP 患者的 EV 内。在女性 CP 患者中发现了一种独特的模式。
更新日期:2021-08-27
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