Ochrobactrum pseudogrignonense (newly named Brucella pseudogrignonensis ) is an emerging pathogen in immunodeficient and immunocompetent patients. Most documented cases associated with Ochrobactrum are frequently catheter-related and exhibit wide-spectrum β-lactam resistance. Misidentification of this pathogen using commercial bacterial identification kits is common. We identified a case of O. pseudogrignonense infection associated with cholelithiasis. The O. pseudogrignonense genome was sequenced and reconstructed using a Nanopore and Illumina hybrid strategy. A novel bla OXA-919 divergent from existing OXA members was identified and subsequent analysis revealed its existence in all available O. pseudogrignonense genomes, which forms a new phylogenetic subgroup distinct from other OXA clusters. Further analysis demonstrated the presence of the novel bla OXA-919 in the chromosome of several other Ochrobactrum species. Our study indicated that Ochrobactrum chromosomes may be a reservoir of bla OXA-919 β-lactamases.

中文翻译：

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Ochrobactrum pseudogrignonense（新命名为 Brucella pseudogrignonensis）的基因组学揭示了一个新的 blaOXA 亚群

Ochrobactrum pseudogrignonense （新命名为Brucella pseudogrignonensis）是免疫缺陷和免疫功能正常患者中的一种新兴病原体。大多数与Ochrobactrum相关的记录病例通常与导管相关，并表现出广谱 β-内酰胺耐药性。使用商业细菌鉴定试剂盒错误鉴定这种病原体很常见。我们发现了一例与胆石症相关的O. pseudogrignonense感染病例。使用 Nanopore 和 Illumina 混合策略对O. pseudogrignonense基因组进行测序和重建。小说bla OXA-919 鉴定出与现有 OXA 成员不同，随后的分析揭示了它存在于所有可用的O. pseudogrignonense基因组中，这形成了一个不同于其他 OXA 簇的新系统发育亚群。进一步的分析表明，在其他几种Ochrobactrum物种的染色体中存在新的bla OXA-919 。我们的研究表明，Ochrobactrum染色体可能是bla OXA-919 β-内酰胺酶的储存库。