Titanium (Ti) and its alloy implants are widely used in the field of orthopedics, and osteoporosis is an important reason for implantation failure. This study aimed to establish a quercetin (QTN) controlled release system on the surface of titanium implants and to study its effects on osteogenesis and osseointegration on the surface of implants. Polyethylenimine (PEI) was first immobilized on a titanium substrate as the base layer, and then, hyaluronic acid/chitosan-quercetin (HA/CS-QTN) multilayer films were assembled on the PEI layer by a self-assembly technique. Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and contact angle measurements were used to characterize and analyze the samples. The release characteristics of QTN were studied by release assays. The osteogenic ability of the samples was evaluated by experiments on an osteoporosis rat model and MC3T3-E1 cells. The FTIR, SEM, and contact angle measurements all showed that the PEI substrate layer and HA/CS-QTN multilayer film were successfully immobilized on the titanium matrix. The drug release test showed the successful establishment of a QTN controlled release system. The in vitro results showed that osteoblasts exhibited higher adhesion, proliferation and differentiation ability on the coated titanium matrix than on the pure titanium surface. In addition, the in vivo results showed that the HA/CS-QTN coating significantly increased the new bone mass around the implant. By depositing a PEI matrix layer and HA/CS-QTN multilayer films on titanium implants, a controlled release system of QTN was established, which improved implant surface osseointegration under osteoporotic conditions. This study proposes a new implant therapy strategy for patients with osteoporosis.

中文翻译：

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通过逐层组装技术在钛表面涂覆载有槲皮素的聚电解质多层，以改善骨质疏松条件下的表面成骨。

钛（Ti）及其合金植入物广泛应用于骨科领域，骨质疏松是植入失败的重要原因。本研究旨在在钛种植体表面建立槲皮素（QTN）控释体系，并研究其对种植体表面成骨和骨整合的影响。首先将聚乙烯亚胺（PEI）固定在钛基底上作为基层，然后通过自组装技术将透明质酸/壳聚糖-槲皮素（HA/CS-QTN）多层膜组装在PEI层上。傅里叶变换红外 (FTIR) 光谱、扫描电子显微镜 (SEM) 和接触角测量用于表征和分析样品。通过释放试验研究了QTN的释放特性。通过对骨质疏松大鼠模型和 MC3T3-E1 细胞的实验评估样品的成骨能力。FTIR、SEM 和接触角测量均表明 PEI 基底层和 HA/CS-QTN 多层膜成功固定在钛基体上。药物释放试验表明QTN控释体系建立成功。这体外实验结果表明，与纯钛表面相比，成骨细胞在涂层钛基质上表现出更高的粘附、增殖和分化能力。此外，体内结果表明，HA/CS-QTN 涂层显着增加了植入物周围的新骨量。通过在钛种植体上沉积 PEI 基质层和 HA/CS-QTN 多层膜，建立了 QTN 控释系统，改善了骨质疏松条件下种植体表面的骨整合。本研究为骨质疏松症患者提出了一种新的种植体治疗策略。