Esophageal cancer is one of the most common human malignancies and ranks sixth for global mortality; the major histological type is esophageal squamous cell carcinoma (ESCC). Here we assessed the effect of long non-coding (lnc) RNA OIP5-AS1 on the miR-30a-5p/Forkhead box protein D1 (FOXD1) axis in ESCC and investigated the underlying mechanism involving the ERK1/2 signaling pathway. lnc RNA OIP5-AS1 was highly expressed in human ESCC tissues and cells, targeted miR-30a-5p, and inhibited miR-30a-5p expression. Additionally, in human ESCC tissues, miR-30a-5p was poorly expressed, whereas FOXD1 mRNA and protein were highly expressed, with a negative correlation between miR-30a-5p and FOXD1 expression. miR-30a-5p targeted and inhibited FOXD1 expression. FOXD1 promoted the proliferation and invasion of ESCC and was related to the ERK1/2 signaling pathway; ERK1/2 inhibitors (LY-3214996) reversed the biological function of FOXD1. miR-30a-5p combined with FOXD1 regulated ERK1/2 expression and inhibited tumor growth in vivo. In this study, micro- and nano-particles were used as carriers to construct Nanocapsules carrying miR-30a-5p mimics and miR-30a-5p inhibitor through self-assembly method, so as to realize an efficient Nanocapsules delivery system of miR-30a-5p to esophageal cancer cells. It provides insights into targeted drug therapy and the development of micro- and nano-particles carriers.

中文翻译：

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长非编码 RNA OIP5-AS1 通过靶向 FOXD1/miR-30a-5p 轴抑制食管鳞状细胞癌细胞的增殖和迁移以及微米和纳米粒子对靶向转染系统的影响。

食道癌是最常见的人类恶性肿瘤之一，在全球死亡率中排名第六；主要的组织学类型是食管鳞状细胞癌（ESCC）。在这里，我们评估了长非编码 (lnc) RNA OIP5-AS1 对 ESCC 中 miR-30a-5p/叉头盒蛋白 D1 (FOXD1) 轴的影响，并研究了涉及 ERK1/2 信号通路的潜在机制。lnc RNA OIP5-AS1在人ESCC组织和细胞中高表达，靶向miR-30a-5p，抑制miR-30a-5p表达。此外，在人 ESCC 组织中，miR-30a-5p 表达量低，而 FOXD1 mRNA 和蛋白质高表达，miR-30a-5p 与 FOXD1 表达呈负相关。miR-30a-5p 靶向并抑制 FOXD1 表达。FOXD1促进ESCC的增殖和侵袭，与ERK1/2信号通路有关；ERK1/2 抑制剂 (LY-3214996) 逆转了 FOXD1 的生物学功能。miR-30a-5p 联合 FOXD1 调节 ERK1/2 表达并抑制肿瘤生长在体内。本研究以微米和纳米粒子为载体，通过自组装的方法构建了携带miR-30a-5p模拟物和miR-30a-5p抑制剂的纳米胶囊，从而实现了高效的miR-30a纳米胶囊递送系统。 -5p 对食管癌细胞。它提供了对靶向药物治疗以及微米和纳米粒子载体开发的见解。