Abstract

Adiponectin is an adipose tissue hormone affecting energy and lipoprotein metabolism and modulating inflammatory responses. However, the role of this adipokine in atherogenesis remains poorly understood. The aim of this study was to investigate the effect of adiponectin on the production of apolipoproteins (apo) A-1 and E by human macrophages (MP). The study was conducted on macrophage-like cells of the THP-1 cell line of two differentiation terms, 3 and 5 days (3d and 5d). Adiponectin (10 μg/mL) stimulated the expression of apoA-1 gene at the mRNA level in 5d MP, but not in 3d MP. The level of apoE mRNA in MP under the action of adiponectin was not affected. Adiponectin suppressed macrophage TNF gene expression, while it induced the expression of IL-10 gene in 5d MP. The secreted levels of apoA-1 and apoE proteins under the action of adiponectin in macrophages of both periods of differentiation remained unchanged, while the level of the surface apoA-1 protein in 5d MP was decreasing. Incubation of 5d MP with the PPARα nuclear receptor antagonist MK-886 or with the nuclear receptor LXR agonist TO-901317 resulted in cancellation of the stimulating effect of adiponectin on apoA-1 gene expression. These data indicate that adiponectin, in addition to its anti-inflammatory action, has a modulating effect on production of apoA-1 by macrophages. The latter is probably one of the mechanisms of the influence of this adipokine on atherogenesis.

中文翻译：

---

脂联素对人巨噬细胞产生载脂蛋白A-1和E的影响

摘要

脂联素是一种脂肪组织激素，影响能量和脂蛋白代谢并调节炎症反应。然而，这种脂肪因子在动脉粥样硬化中的作用仍然知之甚少。本研究的目的是研究脂联素对人巨噬细胞 (MP) 产生载脂蛋白 (apo) A-1 和 E 的影响。该研究是在 3 天和 5 天（3 天和 5 天）两个分化期的 THP-1 细胞系的巨噬细胞样细胞上进行的。脂联素 (10 μg/mL)在 5d MP 中刺激apoA-1基因在 mRNA 水平上的表达，但在 3d MP 中没有。脂联素作用下MP中apoE mRNA水平不受影响。脂联素抑制巨噬细胞TNF基因表达，同时诱导IL-10表达5d MP 中的基因。两个分化时期巨噬细胞在脂联素作用下的apoA-1和apoE蛋白分泌水平均未发生变化，而5d MP表面apoA-1蛋白水平呈下降趋势。5d MP 与 PPARα 核受体拮抗剂 MK-886 或核受体 LXR 激动剂 TO-901317 孵育导致脂联素对apoA-1基因表达的刺激作用消除。这些数据表明，脂联素除了具有抗炎作用外，还对巨噬细胞产生 apoA-1 具有调节作用。后者可能是这种脂肪因子影响动脉粥样硬化的机制之一。