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Long-Chain Free Fatty Acids Influence Lipid Accumulation, Lysosome Activation and Glycolytic Shift in Various Cells In Vitro
Molecular Biology ( IF 1.2 ) Pub Date : 2021-08-26 , DOI: 10.1134/s0026893321030146
K. S. Vishnyakova 1 , K. V. Popov 1 , X. Pan 1 , M. V. Jasko 1 , Y. E. Yegorov 1
Affiliation  

Abstract

Hydrophobic molecules may be toxic when present in excess. When dissolved in membranes, hydrophobic molecules disrupt membrane function. Studies on the effects of free fatty acids (FFA) on cultured cells contradict each other. Here we describe the effects of FFA on various human cells in culture. The addition of long-chain FFA (oleic, palmitic, linoleic, linolenic, etc.) to cultured cells led to lipid accumulation in hepatocytes and muscle cells, initiation of autophagy, and uncoupling of oxidative phosphorylation. Although treated cells increase their oxygen consumption, metabolic shifts in favor of glycolysis were observed. All these effects were expressed to varying degrees in different cells and with the addition of different FFAs. The mechanisms of these FFA effects are discussed, as well their practical implications.



中文翻译:

长链游离脂肪酸影响体外各种细胞的脂质积累、溶酶体激活和糖酵解转变

摘要

当过量存在时,疏水性分子可能是有毒的。当溶解在膜中时,疏水性分子会破坏膜功能。关于游离脂肪酸 (FFA) 对培养细胞影响的研究相互矛盾。在这里,我们描述了 FFA 对培养的各种人类细胞的影响。将长链 FFA(油酸、棕榈酸、亚油酸、亚麻酸等)添加到培养细胞中会导致肝细胞和肌肉细胞中的脂质积累、自噬的启动和氧化磷酸化的解偶联。尽管经过处理的细胞增加了它们的耗氧量,但观察到有利于糖酵解的代谢转变。所有这些影响在不同细胞中和添加不同的 FFA 时都有不同程度的表现。讨论了这些 FFA 效应的机制,以及它们的实际意义。

更新日期:2021-08-27
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