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Decrease in the Activity of Striatal-Enriched Protein-Tyrosine-Phosphatase (STEP) in the Brain of Danio rerio Treated with p-Chlorophenylalanine and Pargyline
Molecular Biology ( IF 1.2 ) Pub Date : 2021-08-26 , DOI: 10.1134/s0026893321020254
E. A. Kulikova 1 , D. V. Fursenko 1 , E. Yu. Bazhenova 1 , A. V. Kulikov 1
Affiliation  

Abstract—

Fundamental neurophysiological processes are often studied using Danio rerio fish as a model. A selective inhibitor of striatal-enriched protein tyrosine phosphatase (STEP) reduces serotonin metabolism in the D. rerio brain. Both STEP and serotonin are involved in the development of neurodegenerative behavioral disorders. Reduction or elevation of the serotonin level in the brain of mice caused by the administration of p-chlorophenylalanine or pargyline, respectively, results in a decrease in the level of рtpn5 mRNA in the striatum, рtpn5 being the gene encoding STEP. However, it has not been established whether this occurs in other organisms. We studied the effect of inhibitors of synthesis (p-chlorophenylalanine) and degradation (pargyline) of serotonin on the expression of the ptpn5 gene and the activity of STEP in the brain of D. rerio. The fish were placed in water containing p-chlorophenylalanine (2 mg/L) or pargyline (0.5 mg/L) for 72 hours, and control subjects were kept in aquarium water. The p-chlorophenylalanine treatment decreased the serotonin level in the brain fourfold, whereas pargyline increased the level of this transmitter sixfold. Both p-chlorophenylalanine and pargyline decrease STEP activity in the D. rerio brain, without affecting the level of the рtpn5 mRNA gene. Thus, interaction between STEP and the serotonin system is observed in both mammals and fish, which indicates the similarity of the regulation processes in vertebrates.



中文翻译:

用对氯苯丙氨酸和 Pargyline 处理的斑马鱼脑中纹状体富集蛋白酪氨酸磷酸酶 (STEP) 活性降低

摘要-

基本的神经生理过程经常使用Danio rerio鱼作为模型进行研究。纹状体富集蛋白酪氨酸磷酸酶 (STEP) 的选择性抑制剂可降低D. rerio大脑中的血清素代谢。STEP 和血清素都参与了神经退行性行为障碍的发展。分别由氯苯丙氨酸或 pargyline给药引起的小鼠脑中血清素水平的降低或升高,导致纹状体中рtpn5 mRNA的水平降低,рtpn5是编码 STEP 的基因。然而,尚未确定这是否发生在其他生物体中。我们研究了合成抑制剂的作用(p-氯苯丙氨酸)和血清素降解(pargyline)对D. rerio脑中ptpn5基因表达和 STEP 活性的影响。将鱼置于含有氯苯丙氨酸(2mg/L)或pargyline(0.5mg/L)的水中72小时,对照组则保持在水族箱水中。所述p -chlorophenylalanine治疗大脑中的四倍血清素水平降低,而增加的优降宁此发射机六倍的水平。无论P-氯苯和优降宁减少步活动在D.鱼脑,在不影响水平рtpn5mRNA 基因。因此,在哺乳动物和鱼类中都观察到 STEP 和血清素系统之间的相互作用,这表明脊椎动物调节过程的相似性。

更新日期:2021-08-27
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