Cyst nematodes are sedentary endoparasites of plants which cause important economic losses worldwide. New nematode control measures are needed since the removal of effective chemical nematicides from the market due to their negative impact on environment and human health. Induced hatching of second-stage juveniles in the absence of host plant using root exudates, also named “suicide hatching”, could be a sustainable alternative method to reduce nematode population densities in infested soils. Unfortunately, biocontrol methods are often less effective in agricultural fields than in laboratory or greenhouse and this could be due to, among other reasons, a different susceptibility of nematode populations to root exudate stimulation. Testing this intra-specific variability would help to anticipate any drop in the efficiency of this new biocontrol strategy. A selection of root exudates from different plant species, maximizing the hatching level highlighted in a previous study, was tested on the hatching of representative populations of the genetic diversity for Globodera pallida, Heterodera schachtii and Heterodera carotae. Results showed significant differences between populations for given nematode species but not correlated with the genetic structure. Overall, root exudates tested provide a high level of hatching of these three nematode species. Surprisingly, the root exudate from broccoli induces hatching of European populations of the potato cyst nematode G. pallida.

囊状线虫是植物体内的久坐寄生虫，在世界范围内造成重大经济损失。由于有效的化学杀线虫剂对环境和人类健康有负面影响，因此需要采取新的线虫控制措施。在没有寄主植物的情况下使用根系分泌物诱导孵化第二阶段幼虫，也称为“自杀孵化”，可能是减少受侵染土壤中线虫种群密度的可持续替代方法。不幸的是，生物防治方法在农田中通常不如在实验室或温室中有效，这可能是由于线虫种群对根系分泌物刺激的不同敏感性等原因。测试这种种内变异将有助于预测这种新生物防治策略效率的任何下降。对来自不同植物物种的根系分泌物的选择，最大限度地提高了先前研究中强调的孵化水平，对遗传多样性的代表性种群的孵化进行了测试Globodera pallida、Heterodera schachtii和Heterodera carotae。结果显示给定线虫物种的种群之间存在显着差异，但与遗传结构无关。总体而言，测试的根系分泌物提供了这三种线虫物种的高水平孵化。令人惊讶的是，西兰花的根系分泌物诱导了欧洲马铃薯胞囊线虫G. pallida种群的孵化。