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Selection of methanotrophic platform for methanol production using methane and biogas
Journal of Bioscience and Bioengineering ( IF 2.8 ) Pub Date : 2021-08-27 , DOI: 10.1016/j.jbiosc.2021.07.007
Pranav Pradip Kulkarni 1 , Vijaykumar Kashinath Khonde 2 , Mandar Sachidanand Deshpande 2 , Tushar Ramdas Sabale 2 , Pramod Shankar Kumbhar 2 , Anand Rameshchandra Ghosalkar 1
Affiliation  

To develop biotechnological process for methane to methanol conversion, selection of a suitable methanotrophic platform is an important aspect. Systematic approach based on literature and public databases was developed to select representative methanotrophs Methylotuvimicrobium alcaliphilum, Methylomonas methanica, Methylosinus trichosporium and Methylocella silvestris. Selected methanotrophs were further investigated for methanol tolerance and methanol production on pure methane as well as biogas along with key enzyme activities involved in methane utilization. Among selected methanotrophs M. alcaliphilum showed maximum methanol tolerance of 6% v/v along with maximum methanol production of 307.90 mg/L and 247.37 mg/L on pure methane and biogas respectively. Activity of methane monooxygenase and formate dehydrogenase enzymes in M. alcaliphilum was significantly higher up to 98.40 nmol/min/mg cells and 0.87 U/mg protein, respectively. Biotransformation trials in 14 L fermentor resulted in increased methanol production up to 418 and 331.20 mg/L, with yield coefficient 0.83 and 0.71 mg methanol/mg of pure methane and biogas respectively. The systematic selection resulted in haloalkaliphilic strain M. alcaliphilum as one of the potential methanotroph for bio-methanol production.



中文翻译:

甲烷和沼气生产甲醇的甲烷营养平台选择

为了开发甲烷到甲醇转化的生物技术工艺,选择合适的甲烷氧化平台是一个重要方面。根据文献和公共数据库系统的方法的开发是为了选择有代表性的甲烷营养生物Methylotuvimicrobium alcaliphilum甲基单methanicaMethylosinus弯菌Methylocella silvestris。进一步研究了选定的甲烷氧化菌对纯甲烷和沼气的甲醇耐受性和甲醇生产以及参与甲烷利用的关键酶活性。在选定的甲烷氧化菌中M. alcaliphilum显示最大甲醇耐受性为 6% v/v,纯甲烷和沼气的最大甲醇产量分别为 307.90 mg/L 和 247.37 mg/L。M. alcaliphilum中甲烷单加氧酶和甲酸脱氢酶的活性显着更高,分别高达 98.40 nmol/min/mg 细胞和 0.87 U/mg 蛋白质。在 14 L 发酵罐中进行的生物转化试验使甲醇产量增加到 418 和 331.20 毫克/升,产量系数分别为 0.83 和 0.71 毫克甲醇/毫克纯甲烷和沼气。系统选择导致嗜碱菌株M. alcaliphilum作为生物甲醇生产的潜在甲烷氧化菌之一。

更新日期:2021-10-24
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