Cromileptes altivelis, humpback grouper, belongs to the family Epinephelidae and is one popular farmed fish species because of its high economic value and ornamental value. However, more and more diseases outbreaks have been reported with C. altivelis aquaculture. Today, a new brain cell line of C. altivelis (named CAB) was established and characterized. Our results showed that CAB cells were suitable for growth at 26 °C in L-15 medium supplemented with 15% fetal bovine serum (FBS). The results of 18S rRNA gene sequencing confirmed that CAB cell line was derived from C. altivelis. Moreover, chromosomal aneuploidy was observed in CAB cells, and the modal chromosome number of CAB cells was 48 by chromosome analysis. In addition, CAB cells could transfect pEGFP-N3 plasmid with high transfection efficiency, indicating that CAB cell line has the potential to investigate the function of exogenous genes in vitro. Furthermore, the bacterial susceptibility results suggested that CAB cells were susceptive to Vibrio harveyi and Edwardsiella tarda. And, heavy metals (Hg, Cd, and Cu) were toxic to the CAB cells, and the toxic effect was dose-dependent. In summary, the CAB cell line could be a powerful tool in vitro to study functional genes and has the potential application in bacterial susceptibility and toxicology.

Cromileptes altivelis，座头斑鱼，属于石斑鱼科，具有较高的经济价值和观赏价值，是一种受欢迎的养殖鱼类。然而，随着C. altivelis水产养殖的爆发，越来越多的疾病爆发。今天，一种新的C. altivelis脑细胞系（命名为 CAB）被建立和表征。我们的结果表明，CAB 细胞适合在补充有 15% 胎牛血清 (FBS) 的 L-15 培养基中在 26°C 下生长。18S rRNA基因测序结果证实CAB细胞系来源于C. altivelis. 此外，在CAB细胞中观察到染色体非整倍性，染色体分析显示CAB细胞的模态染色体数为48。此外，CAB细胞能够以高转染效率转染pEGFP-N3质粒，表明CAB细胞系具有体外研究外源基因功能的潜力。此外，细菌易感性结果表明 CAB 细胞对哈维弧菌和迟发爱德华氏菌敏感。并且，重金属（Hg、Cd 和 Cu）对 CAB 细胞具有毒性，并且毒性作用呈剂量依赖性。总之，CAB 细胞系可以成为体外研究功能基因的有力工具，并在细菌敏感性和毒理学方面具有潜在的应用价值。