It was found that the modification of one side of lipid membranes by amphotericin B and N‑methyl derivatives of amphotericin B (methamphocin) resulted in a discrete increase in the membrane conductivity by the channel mechanism. The conditions under which amphotericin B increased the conductivity of membranes upon addition on one side of the membranes were found. The effect of amphotericin B upon addition on one side of the membranes was observed in an acidic medium (pH 3.0) and at a two-fold lower concentration of phospholipids in the membrane-forming solution. A large dispersion of the conductivity from 2 to 20 pS of single channels was revealed. The channels with the conductivity of 10 pS were most likely to occur. The histogram of distribution of the conductivity of metamphocin channels showed that the channels with the conductivity of 5 pS were most likely to occur. The selective permeability of membranes upon addition of methamphocin on one side of the membranes was predominantly anionic and did not depend on the concentration of cholesterol in the membranes. The mechanism of the amphotericin B and methamphocin action from one side of the membranes was due to the formation of semipores in the membranes, which were asymmetric in their structure. It was assumed that the selective permeability of the amphotericin and metamphocin channels was determined by the molecular structure of the hydrophilic chain that lines the inner cavity of the semipore.

研究发现，两性霉素 B 和两性霉素 B 的 N-甲基衍生物（甲基安培霉素）对脂质膜一侧的修饰通过通道机制导致膜电导率的离散增加。发现了在膜的一侧添加后两性霉素 B 增加膜电导率的条件。在酸性介质 (pH 3.0) 和成膜溶液中磷脂浓度低两倍的情况下，观察到两性霉素 B 添加到膜的一侧后的影响。揭示了单通道从 2 到 20 pS 的电导率的大分散。电导率为 10 pS 的通道最有可能出现。甲氨蝶呤通道电导率分布直方图表明，电导率为5 pS的通道最有可能出现。在膜的一侧加入甲安霉素后，膜的选择性渗透性主要是阴离子性的，并不取决于膜中胆固醇的浓度。膜一侧的两性霉素 B 和甲氨蝶呤作用机制是由于膜中形成了结构不对称的半孔。假设两性霉素和甲基安福霉素通道的选择性渗透性是由排列在半孔内腔的亲水链的分子结构决定的。在膜的一侧加入甲安霉素后，膜的选择性渗透性主要是阴离子性的，并不取决于膜中胆固醇的浓度。膜一侧的两性霉素 B 和甲氨蝶呤作用机制是由于膜中形成了结构不对称的半孔。假设两性霉素和甲基安福霉素通道的选择性渗透性是由排列在半孔内腔的亲水链的分子结构决定的。在膜的一侧加入甲安霉素后，膜的选择性渗透性主要是阴离子性的，并不取决于膜中胆固醇的浓度。膜一侧的两性霉素 B 和甲氨蝶呤作用机制是由于膜中形成了结构不对称的半孔。假设两性霉素和甲基安福霉素通道的选择性渗透性是由排列在半孔内腔的亲水链的分子结构决定的。它们的结构是不对称的。假设两性霉素和甲基安福霉素通道的选择性渗透性是由排列在半孔内腔的亲水链的分子结构决定的。它们的结构是不对称的。假设两性霉素和甲基安福霉素通道的选择性渗透性是由排列在半孔内腔的亲水链的分子结构决定的。