The effects of mono- and disubstituted phosphates on the photolysis reaction of reduced nicotinamide adenine dinucleotide (NADH) in a frozen (77 K) aqueous solution were studied by EPR. The dependence of the yield of the paramagnetic intermediates of the reaction, that is, the NADH⁺ cation radical and atomic hydrogen, on the concentration of phosphates was determined. The results were interpreted from the point of view of the basicity and electron acceptor properties of phosphates. Electron spectroscopy data revealed a significant acceleration of auto-oxidation and photoinduced oxidation of NADH under normal conditions (20°C, aqueous medium, phosphate buffer, pH 6.3). The rate of the dark reaction was directly proportional to the total concentration of orthophosphates and hydrophosphates and was insensitive to oxygen. On the basis of experiments with a nitroxyl trap, the conclusion was made that the reaction is not of a radical nature.

通过 EPR 研究了单和二取代磷酸盐对冷冻 (77 K) 水溶液中还原烟酰胺腺嘌呤二核苷酸 (NADH) 光解反应的影响。反应顺磁性中间体产率的依赖性，即 NADH ⁺阳离子自由基和原子氢，对磷酸盐的浓度进行了测定。从磷酸盐的碱度和电子受体性质的角度解释结果。电子光谱数据显示，在正常条件下（20°C，水性介质，磷酸盐缓冲液，pH 6.3），NADH 的自氧化和光诱导氧化显着加速。暗反应的速率与正磷酸盐和氢磷酸盐的总浓度成正比，并且对氧不敏感。根据用硝酰基捕集器进行的实验，得出的结论是该反应不是自由基性质的。