Embryo development in eggs of the spittlebug Mahanarva spectabilis (Distant) (Hemiptera: Cercopidae) passes through four phases (known as S1 to S4) being stopped at S2 during diapause. Studies about the molecular basis of diapause in spittlebugs are nonexistent. Here, we analyzed proteins from non-diapausing (ND), diapausing (D) and post-diapausing (PD) eggs of the spittlebug M. spectabilis. In total, we identified 87 proteins where 12 were in common among the developmental and diapause phases and 19 remained as uncharacterized. Non-diapausing eggs (S2ND and S4ND) showed more proteins involved in information storage and processing than the diapausing ones (S2D). Eggs in post-diapausing (S4PD) had a higher number of proteins associated with metabolism than S2D. The network of protein interactions and metabolic processes allowed the identification of different sets of molecular interactions for each developmental and diapause phases. Two heat shock proteins (Hsp65 and Hsp70) along with two proteins associated with intracellular signaling (MAP4K and a serine/threonine-protein phosphatase) were found only in diapausing and/or post-diapausing eggs and are interesting targets to be explored in future experiments. These results shine a light on one key biological process for spittlebug survival and represent the first search for proteins linked to diapause in this important group of insects.

在滞育期间在 S2 停止的 Spittlebug Mahanarva spectabilis (Distant) (Hemiptera: Cercopidae)卵中的胚胎发育经历了四个阶段（称为 S1 到 S4）。不存在关于臭虫滞育的分子基础的研究。在这里，我们分析了来自 Spittlebug M. spectabilis的非滞育 (ND)、滞育 (D) 和滞育后 (PD) 卵的蛋白质. 总共，我们确定了 87 种蛋白质，其中 12 种在发育和滞育阶段是共同的，19 种仍然没有被表征。非滞育卵（S2ND 和 S4ND）显示出比滞育卵（S2D）更多的参与信息存储和处理的蛋白质。滞育后 (S4PD) 中的卵子与新陈代谢相关的蛋白质数量高于 S2D。蛋白质相互作用和代谢过程的网络允许识别每个发育和滞育阶段的不同分子相互作用集。仅在滞育和/或滞育后的卵中发现了两种热休克蛋白（Hsp65 和 Hsp70）以及两种与细胞内信号传导相关的蛋白（MAP4K 和丝氨酸/苏氨酸蛋白磷酸酶），它们是未来实验中值得探索的有趣目标.