In this study, an optimized procedure of sample preparation for quantitative determination of the antiviral agent camphecene in dried rat blood spots was developed. It has been shown that when using methanol containing 0.1% HCOOH as an extractant, the recovery of the substance increases in comparison with the previously developed method. In addition to this, there is no need to dilute the obtained solutions with water for the analysis of the sample by high-performance liquid chromatography (HPLC) on a column with a reversed-phase sorbent. By using the developed method, the stability of samples of dried rat blood spots containing camphecene in different concentrations at different temperatures was studied. It was found that while the samples were stored at room temperature, apparently, desorption of the substance occurs leading to a loss of more than 15% of its initial amount after 5–10 days. Lowering the temperature increases the stability of samples and their storage at −70°C is possible for 4 weeks.

中文翻译：

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不同温度下抗病毒剂樟脑在干血斑中的稳定性研究

在这项研究中，开发了一种优化的样品制备程序，用于定量测定干大鼠血斑中的抗病毒剂樟脑。已经表明，当使用含有 0.1% HCOOH 的甲醇作为萃取剂时，与以前开发的方法相比，该物质的回收率有所提高。除此之外，不需要用水稀释获得的溶液，以便在具有反相吸附剂的柱上通过高效液相色谱 (HPLC) 分析样品。利用所开发的方法，研究了含有不同浓度樟脑烯的干大鼠血斑样品在不同温度下的稳定性。结果发现，虽然样品在室温下储存，但显然，物质发生解吸，导致 5-10 天后损失超过其初始量的 15%。降低温度可以提高样品的稳定性，并且可以在 -70°C 下储存 4 周。