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Meldonium residues in milk: A possible scenario for inadvertent doping in sports?
Drug Testing and Analysis ( IF 2.9 ) Pub Date : 2021-08-26 , DOI: 10.1002/dta.3145 Sven Guddat 1 , Christian Görgens 1 , Tim Sobolevsky 2 , Mario Thevis 1
Drug Testing and Analysis ( IF 2.9 ) Pub Date : 2021-08-26 , DOI: 10.1002/dta.3145 Sven Guddat 1 , Christian Görgens 1 , Tim Sobolevsky 2 , Mario Thevis 1
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Lately, the veterinary drug Emidonol® has been discussed as a possible scenario for inadvertent doping in sports. Emidonol® is approved for use in livestock breeding, exhibiting antihypoxic and weak sedative effects. The veterinary drug rapidly dissociates into meldonium, a substance prohibited in sports, and is excreted largely in its unchanged form into urine. To investigate if residues of meldonium in edible produce may result in adverse analytical findings in sports drug testing, a pilot study was conducted with three volunteers consuming a single dose of 100 ml meldonium-spiked milk at a concentration of 500 ng/ml (Study 1), and multiple doses of 100 ml of meldonium-spiked milk (500 ng/ml) on five consecutive days (Study 2). In the single dose study, urinary meldonium concentrations peaked between 2 and 6 h post-administration with maximum values of 7.5 ng/ml, whereas maximum meldonium concentrations of 18.6 ng/ml were determined after multiple doses 4 h post-administration. All samples were analyzed using an established and validated protocol based on HILIC-HRMS/MS.
中文翻译:
牛奶中的米屈肼残留:运动中不慎使用兴奋剂的可能情况?
最近,兽药 Emidonol® 已被讨论为运动中无意使用兴奋剂的一种可能情况。Emidonol® 被批准用于畜牧养殖,具有抗缺氧和弱镇静作用。该兽药迅速分解为运动中禁止使用的物质米屈肼,大部分以原形排泄到尿液中。为了调查食用产品中的米屈肼残留是否会导致运动药物测试中的不良分析结果,一项试点研究由三名志愿者进行,他们食用单剂量的 100 毫升浓度为 500 纳克/毫升的米屈肼加标牛奶(研究 1 ),以及连续五天多次服用 100 毫升米屈肼加标牛奶 (500 ng/ml)(研究 2)。在单剂量研究中,尿米曲肼浓度在给药后 2 至 6 小时达到峰值,最大值为 7.5 ng/ml,而在给药后 4 小时多次给药后测定的最大米曲肼浓度为 18.6 ng/ml。所有样品均使用基于 HILIC-HRMS/MS 的已建立且经过验证的协议进行分析。
更新日期:2021-08-26
中文翻译:
牛奶中的米屈肼残留:运动中不慎使用兴奋剂的可能情况?
最近,兽药 Emidonol® 已被讨论为运动中无意使用兴奋剂的一种可能情况。Emidonol® 被批准用于畜牧养殖,具有抗缺氧和弱镇静作用。该兽药迅速分解为运动中禁止使用的物质米屈肼,大部分以原形排泄到尿液中。为了调查食用产品中的米屈肼残留是否会导致运动药物测试中的不良分析结果,一项试点研究由三名志愿者进行,他们食用单剂量的 100 毫升浓度为 500 纳克/毫升的米屈肼加标牛奶(研究 1 ),以及连续五天多次服用 100 毫升米屈肼加标牛奶 (500 ng/ml)(研究 2)。在单剂量研究中,尿米曲肼浓度在给药后 2 至 6 小时达到峰值,最大值为 7.5 ng/ml,而在给药后 4 小时多次给药后测定的最大米曲肼浓度为 18.6 ng/ml。所有样品均使用基于 HILIC-HRMS/MS 的已建立且经过验证的协议进行分析。