The effects of continuous lighting (CL) before gene transfer on leaf biomass, leaf hemagglutinin (HA) content per biomass, and leaf HA content per plant in Nicotiana benthamiana after gene transfer were investigated in a transient gene expression system. A control photoperiod of 16 h d^-1 and an extended photoperiod of 24 h d^-1 were compared at two photosynthetic photon flux density (PPFD) levels of 200 and 400 µmol m^-2 s^-1. Leaf biomass was greater in the 24 h d^-1 photoperiod treatments and 400 µmol m^-2 s^-1 PPFD treatments. The leaf HA content per biomass was smaller at 24 h d^-1 than at 16 h d^-1, indicating that CL before gene transfer had a negative impact on leaf HA accumulation per biomass after gene transfer. There was no significant difference in leaf HA content per biomass between 200 and 400 µmol m^-2 s^-1 PPFD. As a result, the leaf HA content per plant was significantly greater in the high PPFD treatments but not significantly different between photoperiod treatments. The results indicate that a simple approach of applying CL before gene transfer increases biomass but not the HA yield and is thus not necessarily cost-effective in the plant-based recombinant protein production system.

中文翻译：

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基因转移前的 24 小时光周期促进生物量产生，但不促进本氏烟草中瞬时表达的流感血凝素的产量

在瞬时基因表达系统中研究了基因转移前连续光照 (CL) 对基因转移后本氏烟草叶片生物量、每个生物量的叶血凝素 (HA) 含量和每株植物的叶 HA 含量的影响。在 200 和 400 µmol m ^-2 s ^{-1 的}两个光合光子通量密度 (PPFD) 水平下比较了 16 hd ^{-1 的}对照光周期和 24 hd ^-1的延长光周期。24 hd ^-1光周期处理和400 µmol m ^-2 s ^-1 PPFD 处理的叶片生物量更大。每个生物量的叶 HA 含量在 24 hd ^{-1 时}小于 16 hd^-1，表明基因转移前的 CL 对基因转移后每个生物量的叶 HA 积累具有负面影响。在 200 和 400 µmol m ^-2 s ^-1 PPFD之间，每个生物量的叶 HA 含量没有显着差异。因此，在高 PPFD 处理中，每株植物的叶 HA 含量显着更高，但在光周期处理之间没有显着差异。结果表明，在基因转移之前应用 CL 的简单方法会增加生物量，但不会增加 HA 产量，因此在基于植物的重组蛋白生产系统中不一定具有成本效益。