Green synthesis of nanoparticles using plant extract is a novel substitute for conventional chemical or physical methods. A simple green synthesis of pure and Ag-doped tin oxide nanoparticles from the fruit extract of Averrhoa bilimbi and their antibacterial activities have been demonstrated in this work. Silver has been doped into tin oxide for a perceived enhanced antibacterial activity. Techniques like UV-DRS, XRD, FTIR, and TEM were used to characterize the green synthesized nanoparticles. An average crystalline particle size of 2.6 nm was observed in XRD studies. Apart from the structural elucidation, the zeta potential values of the particles were also done. The antibacterial activity of pure and doped nanoparticles against the bacterial leaf blight pathogen, Xanthomonas oryzae was tested adopting standard assays employed in antimicrobial analysis. A significant advantage of using the biosynthesized SnO₂ and its silver-doped variant was noticed in all the assays carried out. The impact of nanoparticle in the cellular protein leakage and the stress-induced enzyme release was carried out using Bradford’s method and DPPH assay respectively. Based on the biofilm inhibition studies, the inhibitory effect of the nanoparticles towards xanthomonadin and exopolysaccharide formation was reaffirmed. The DNA cleavage analysis carried out also was found to be a proof of the bactericidal effect of the nanoparticles. Overall results point to a promising use of Ag-doped SnO₂ nanoparticles against the leaf blight disease caused by Xanthomonas oryzae in rice.

使用植物提取物绿色合成纳米颗粒是传统化学或物理方法的新替代品。在这项工作中已经证明了从Averrhoa bilimbi的果实提取物中简单地绿色合成纯的和掺银的氧化锡纳米粒子及其抗菌活性。银已被掺杂到氧化锡中以增强抗菌活性。使用 UV-DRS、XRD、FTIR 和 TEM 等技术来表征绿色合成的纳米粒子。在XRD研究中观察到2.6nm的平均结晶粒度。除了结构解析外，还进行了粒子的zeta电位值。纯纳米颗粒和掺杂纳米颗粒对细菌性叶枯病病原体米黄单胞菌的抗菌活性采用抗菌分析中采用的标准测定法进行了测试。在进行的所有分析中都注意到使用生物合成的 SnO ₂及其掺杂银的变体的显着优势。分别使用 Bradford 法和 DPPH 测定法进行纳米颗粒对细胞蛋白质渗漏和应激诱导的酶释放的影响。基于生物膜抑制研究，纳米颗粒对黄单胞菌素和胞外多糖形成的抑制作用得到重申。还发现进行的 DNA 切割分析证明了纳米颗粒的杀菌作用。总体结果表明，掺 Ag 的 SnO ₂纳米颗粒有望用于防治米黄单胞菌引起的叶枯病 在米饭中。