Colombia is the largest worldwide producer of Cape gooseberry (Physalis peruviana L.), which is an exotic tropical fruit with increasing high demand in international markets. Unfortunately, the rapid expansion of this crop has occurred without implementing adequate surveillance measures that enforce the use of healthy planting material for the establishment of new crops. This study investigated the prevalence of viruses in P. peruviana using seeds, plantlets, and adult plants with or without symptoms typical of viral infection in the province of Antioquia. Detection of viruses by RT-qPCR revealed widespread infection with potato virus Y (PVY), potato virus X (PVX), potato virus S (PVS), Cape gooseberry ilarvirus 1 (CGIV-1), and Physalis vein necrosis virus (PhyVNV), a proposed new nepovirus species. Co-infections were also common in all samples tested. Further high-throughput sequencing analysis confirmed the presence of most of these viruses and revealed the presence of a new torradovirus (Physalis torrado virus, PhyTV), a new potexvirus (Physalis virus X, PhyVX), and a divergent tobacco mosaic virus isolate. These results reveal that the P. peruviana virome is largely unexplored, and urgent measures should be implemented to certify the health status of planting material, and crops.

哥伦比亚是世界上最大的醋栗 ( Physalis peruviana L.) 生产国，这是一种异国情调的热带水果，在国际市场上的需求量越来越大。不幸的是，这种作物的快速扩张并没有实施足够的监督措施，强制使用健康的种植材料来种植新作物。本研究调查了秘鲁假单胞菌中病毒的流行情况使用在安蒂奥基亚省有或没有典型病毒感染症状的种子、幼苗和成年植物。通过 RT-qPCR 检测病毒显示马铃薯病毒 Y (PVY)、马铃薯病毒 X (PVX)、马铃薯病毒 S (PVS)、角醋栗 ilarvirus 1 (CGIV-1) 和酸浆静脉坏死病毒 (PhyVNV) 广泛感染, 一种提议的新 nepovirus 物种。合并感染在所有测试样本中也很常见。进一步的高通量测序分析证实了大多数这些病毒的存在，并揭示了一种新的龙卷风病毒（Physalis torradovirus，PhyTV）、一种新的potexvirus（Physalisvirus X，PhyVX）和一种不同的烟草花叶病毒分离株的存在。这些结果表明，P. peruviana 病毒组在很大程度上尚未得到探索，应采取紧急措施来证明种植材料和作物的健康状况。