Background and objectives: The emergence of multi-drug resistant (MDR) strains of Plasmodium falciparum highlights the need to develop novel antimalarial drugs. Present study explores the in vivo antiplasmodial activity of ethanol leaf extract of Thalictrum foliolosum (ELETF) against lethal murine malaria.
Methods: The acute toxicity of the extract was assessed by Limit test of Lorke. The suppressive activity of the extract was evaluated by Peter’s 4 day test. In vivo preventive and curative activity of ELETF was assessed by Peter’s method and Ryley and Peter’s method respectively. Biochemical assays were carried out using standard methods.
Results: ELETF (1000 mg/kg) exhibited considerable in vivo schizontocidal activity with 67.11% chemosuppression on Day 5. The ED₅₀ of the extract was 579.56 mg/kg. ELETF also showed significant repository activity with 87.70% chemosuppression at 750 mg/kg, which was greater than pyrimethamine (78.78%). ELETF exhibited dose dependent chemosuppression in the curative test with maximum 70.06% chemosuppression (750 mg/kg). Maximum Mean Survival Time (MST) was 19.2±4.60 and 22.66±4.41 days respectively in the suppressive and curative test, which was extremely statistically significant (p<0.0005) in comparison to untreated control which died by Day 9 post inoculation. Biochemical analysis revealed the safety of ELETF to the hepatic and renal functions of the rodent host.
Interpretation & conclusion: The study reports the antiplasmodial potential of Thalictrum foliolosum (ELETF) against Plasmodium berghei infection. The extract can be developed as a phytomedicine against malaria. Alternatively, the active components can be isolated as new lead compounds against the disease.

中文翻译：

---

Thalictrum foliolosum（毛茛科）对致死性鼠疟疾的抗疟原虫潜力

背景和目的：恶性疟原虫多重耐药 (MDR) 菌株的出现凸显了开发新型抗疟药物的必要性。本研究探讨了Thalictrum foliolosum (ELETF)的乙醇叶提取物对致死性鼠疟疾的体内抗疟原虫活性。方法：通过Lorke极限试验评价提取物的急性毒性。提取物的抑制活性通过 Peter 的 4 天测试进行评估。ELETF的体内预防和治疗活性分别通过Peter's方法和Ryley和Peter's方法进行评估。使用标准方法进行生化测定。结果：

ELETF (1000 mg/kg)在第 5 天表现出相当大的体内裂殖体杀灭活性，化学抑制率为 67.11%。提取物的 ED ₅₀为 579.56 mg/kg。ELETF 还显示出显着的储存库活性，750 mg/kg 的化学抑制率为 87.70%，高于乙胺嘧啶 (78.78%)。ELETF 在治愈试验中表现出剂量依赖性化学抑制，最大化学抑制率为 70.06% (750 mg/kg)。在抑制和治愈试验中，最大平均存活时间 (MST) 分别为 19.2±4.60 和 22.66±4.41 天，与在接种后第 9 天死亡的未处理对照相比，具有极其显着的统计学意义 (p<0.0005)。生化分析揭示了 ELETF 对啮齿动物宿主的肝肾功能的安全性。
解释和结论：该研究报告了Thalictrum foliolosum (ELETF) 对伯氏疟原虫感染的抗疟原虫潜力。该提取物可开发为抗疟疾的植物药。或者，可以将活性成分作为针对该疾病的新先导化合物进行分离。