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Molecular typing of dengue viruses circulating in Assam, India during 2016–2017
Journal of Vector Borne Diseases ( IF 0.5 ) Pub Date : 2020-07-01 , DOI: 10.4103/0972-9062.311779
A Sharma 1 , G Rajbongshi 1 , S T Alam 1 , D Rabha 1 , K Chamuah 1 , L N Henbi 1 , B Borah 1
Affiliation  

Background & objectives: The global incidence of dengue has grown dramatically in recent decades and Assam, India has witnessed several outbreaks of dengue since 2015. Although during post-monsoon months (September to December), most cases of dengue in Assam are recorded but incidence of dengue in Assam has been slowly changing from being endemic to being hyper endemic. Therefore, this study was carried out to determine the serotypes and genotypes of dengue virus prevalent in Assam during the period of 2016–2017.
Methods: This is a prospective study conducted for a period of two years from 2016 to 2017. Department of Microbiology, Gauhati Medical College and Hospital (GMCH) had received a total of ~12000 and ~9000 sera sample during 2016 and 2017 respectively for confirmation of clinically suspected dengue cases. For confirmation, dengue NS1 antigen and IgM antibody ELISA tests were performed. Multiplex RT-PCR was performed for serotyping of dengue viruses and representative samples found positive in PCR were sequenced to determine the genotypes of circulating dengue virus serotypes.
Results: In the year 2016, 6157 sera samples and in 2017, 3386 sera samples were found positive in ELISA test. A total of 157 dengue positive sera samples representing 17 districts of Assam were further tested by multiplex RT-PCR for serotyping of the virus. In PCR, out of 157, 107 samples (68.15%) were found positive for the presence of dengue virus genome. Out of 107, 74 samples (69.15%) were positive for dengue virus serotype-1 (DENV-1), 32 samples (29.90%) for dengue virus serotype-2 (DENV-2) and one sample (0.93%) positive for dengue virus serotype-3 (DENV-3). Out of 107 PCR positive samples, 25 samples were sequenced to identify their genotypes. Phylogenetic analysis of sequenced dengue viruses revealed that all the seven DENV-1 strains were genotype V, 17 DENV-2 strains were genotype IV (Cosmopolitan genotype) and one DENV-3 strain was genotype III.
Interpretation & conclusion: These findings improve our knowledge of circulating dengue virus serotypes in Assam. Co-circulation of three serotypes of dengue virus highlights the need for establishment of active dengue surveillance. The genotypic data of our findings will be helpful for future dengue molecular epidemiology studies and to control the disease in the region.


中文翻译:

2016-2017 年印度阿萨姆邦登革热病毒的分子分型

背景与目标:近几十年来,全球登革热发病率急剧上升,印度阿萨姆邦自 2015 年以来见证了数次登革热暴发。阿萨姆邦的登革热正在缓慢地从地方性转变为高度地方性。因此,本研究旨在确定 2016-2017 年期间阿萨姆邦流行的登革热病毒的血清型和基因型。
方法:这是一项从 2016 年到 2017 年为期两年的前瞻性研究。 高哈蒂医学院和医院 (GMCH) 微生物学系在 2016 年和 2017 年分别收到了约 12000 份和约 9000 份血清样本,用于临床确认疑似登革热病例。为了确认,进行了登革热 NS1 抗原和 IgM 抗体 ELISA 测试。对登革热病毒的血清型进行多重 RT-PCR,并对 PCR 中发现阳性的代表性样本进行测序,以确定循环登革热病毒血清型的基因型。
结果:2016年有6157份血清样本,2017年有3386份血清样本在ELISA检测中呈阳性。代表阿萨姆邦 17 个区的总共 157 份登革热阳性血清样本进一步通过多重 RT-PCR 进行了病毒血清分型检测。在 PCR 中,在 157 个样本中,有 107 个样本 (68.15%) 发现登革热病毒基因组呈阳性。在 107 个样本中,74 个样本 (69.15%) 对登革热病毒血清型 1 (DENV-1) 呈阳性,32 个样本 (29.90%) 对登革热病毒血清型 2 (DENV-2) 呈阳性,1 个样本 (0.93%) 呈阳性登革热病毒血清型 3 (DENV-3)。在 107 个 PCR 阳性样本中,对 25 个样本进行了测序以确定它们的基因型。登革热病毒序列的系统发育分析表明,所有 7 株 DENV-1 毒株均为基因型 V,17 株 DENV-2 毒株为基因型 IV(Cosmopolitan 基因型),1 株 DENV-3 毒株为基因型 III。
解释和结论:这些发现提高了我们对阿萨姆邦登革热病毒血清型的了解。三种血清型登革热病毒的共同传播凸显了建立主动登革热监测的必要性。我们研究结果的基因型数据将有助于未来登革热分子流行病学研究和控制该地区的疾病。
更新日期:2020-07-01
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