In the present study, the antimicrobial and antibiofilm activities of two 4,5-dihydro-1H-pyrazole-1-carboximidamide hydrochloride, (trifluoromethyl) phenyl-substituted (compound 1) and bromophenyl-substituted (compound 2), were evaluated against four Salmonella spp. serotypes through broth microdilution and biofilm-forming activity. Further, the cytotoxicity of the compounds was evaluated by cell viability assays using cultures of HeLa and Vero cell lines, and the mutagenic potential was assessed by the Ames test. In the broth microdilution test, compound 1 inhibited 90% of the strains tested at the minimum inhibitory concentration of 62.5 μg mL⁻¹. Furthermore, both compounds prevented biofilm formation, with a reduction of up to 5.2 log₁₀. HeLa and Vero cells exhibited 100% viability in the presence of compound 1. In contrast, low cell viability was observed in the presence of 15 µg mL⁻¹ of compound 2. Furthermore, no mutagenic potential was detected at any of the tested concentrations of compound 1.

中文翻译：

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4,5-Dihydro-1H-pyrazole-1-carboximidamide Hydrochloride 对沙门氏菌的抗菌和抗生物膜活性。

在本研究中，评估了两种 4,5-二氢-1H-吡唑-1-羧酰亚胺盐酸盐、（三氟甲基）苯基取代（化合物1）和溴苯基取代（化合物2）的抗菌和抗生物膜活性。沙门氏菌属 通过肉汤微量稀释和生物膜形成活性检测血清型。此外，通过使用 HeLa 和 Vero 细胞系培养物的细胞活力测定来评估化合物的细胞毒性，并通过 Ames 测试评估诱变潜力。在肉汤微量稀释试验中，化合物1在 62.5 μ g mL ^-1的最小抑制浓度下抑制了 90% 的试验菌株 . 此外，这两种化合物均可防止生物膜形成，减少高达 5.2 log ₁₀。HeLa 和 Vero 细胞在化合物1存在下表现出 100% 的活力。相比之下，在15 μ g mL ^-1的化合物2存在下观察到低细胞活力 。此外，在化合物1的任何测试浓度下均未检测到诱变潜力。