Per- and polyfluoroalkyl substances (PFAS) are a group of synthetic chemicals detected throughout the environment. To better understand the distribution of PFAS in an aquatic system (the Laurentian Great Lakes), stable isotope enrichment (δ¹³C and δ¹⁵N), fatty acid (FA) profiles, and PFAS were measured in various species from the Lake Ontario (LO) aquatic food web. Sampled organisms included top predator fish, prey fish, and benthic and pelagic macroinvertebrates. The trophic level of each species in the LO food web was determined using δ¹⁵N, and FA concentrations (range: <1–139 mg/g wet weight (ww)). The individual PFAS concentrations in the LO food web were ~1.5 to 5 times lower than previously reported. The highest PFAS concentrations were observed in deepwater sculpin (Myoxocephalus thompsonii, 150 ± 35.7 ng/g ww) suggesting a potential source of PFAS from the offshore benthic zone or sediment. The concentration of PFOS and long chain (C9-C14) perfluoroalkyl carboxylic acids (PFCAs) were significantly higher than short chain PFAS indicating the significant impact of hydrophobicity on the bioaccumulation of PFAS in organisms from the food web. However, high molecular weight PFCAs (>C8) did not exhibit increasing biomagnification factors (BMFs) and trophic magnification factors (TMFs) with log K_ow, suggesting hydrophobicity does not govern the movement of PFAS from low to high trophic levels in the LO food web.

全氟和多氟烷基物质 (PFAS) 是在整个环境中检测到的一组合成化学品。为了更好地了解 PFAS 在水生系统（劳伦森五大湖）中的分布，我们测量了安大略湖不同物种的稳定同位素富集度（δ ¹³ C 和 δ ¹⁵ N）、脂肪酸 (FA) 谱和 PFAS。 LO) 水生食物网。采样的生物包括顶级捕食鱼、猎物鱼以及底栖和中上层大型无脊椎动物。LO 食物网中每个物种的营养水平使用 δ15^确定 N 和 FA 浓度（范围：<1–139 mg/g 湿重 (ww)）。LO 食物网中的单个 PFAS 浓度比先前报道的低约 1.5 至 5 倍。在深水杜宾鱼（ Myoxocephalus thompsonii ，150 ± 35.7 ng/g ww）中观察到最高的 PFAS 浓度，这表明 PFAS 的潜在来源来自近海底栖带或沉积物。全氟辛烷磺酸和长链 (C9-C14) 全氟烷基羧酸(PFCA) 的浓度显着高于短链全氟辛烷磺酸，表明疏水性对全氟辛烷磺酸在食物网生物体内的生物积累具有显着影响。然而，高分子量 PFCA (>C8) 并没有表现出随着_{log K ow增加}的生物放大因子 (BMFs) 和营养放大因子 (TMFs)，表明疏水性不控制 PFAS 在 LO 食物网中从低营养水平到高营养水平的运动。