Objective

Resin-modified glass ionomer cements (RMGICs) present several advantages (e.g. fluoride release), but their reported cytotoxicity has been associated with hydroxyethyl methacrylate (HEMA) monomer release. Therefore, different monomers were tested for use in RMGICs in order to improve their biocompatibility and reduce monomer release.

Methods

Eight experimental liquid compositions were prepared replacing different percentages of HEMA (conventional monomer used in commercial RMGICs) with hydroxypropyl-methacrylate (HPM) and/or tetrahydrofurfuryl-methacrylate (THFM), which are known to have better biocompatibility. Moreover, two commercial materials (Fuji-Plus and RelyX) and two compositions, based on these (home), were included as controls. Monomer release of all materials (commercial, home and experimental) were tested using high-performance liquid chromatography (HPLC) methods after immersing discs in deionized-water (DW) or ethanol:DW. Cytotoxicity of the materials extracts was tested on normal human oral fibroblast line (NHOF-1) using 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay.

Results

Three experimental materials containing THFM (F3, R3 and R4) showed less or similar monomer release compared to corresponding commercial products. Furthermore, two experimental materials (F3 and F4) showed similar effects on NHOF-1 cells compared to the negative control medium.

Significance

The lower monomer release and higher cell viability of some experimental THFM compositions are encouraging. THFM partially replacing HEMA is potentially a suitable alternative for producing biocompatible RMGICs.

中文翻译：

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实验性树脂改性玻璃离子水门汀的特性，含有 HEMA 的替代单体

客观的

树脂改性玻璃离聚物粘固剂 (RMGIC) 具有多种优势（例如释放氟化物），但据报道其细胞毒性与甲基丙烯酸羟乙酯 (HEMA) 单体释放有关。因此，测试了不同单体在 RMGIC 中的使用，以提高它们的生物相容性并减少单体释放。

方法

制备了八种实验液体组合物，用甲基丙烯酸羟丙酯 (HPM) 和/或甲基丙烯酸四氢糠酯 (THFM) 代替不同百分比的 HEMA（商业 RMGIC 中使用的常规单体），已知它们具有更好的生物相容性。此外，包括两种商业材料（Fuji-Plus 和 RelyX）和基于这些材料（家庭）的两种组合物作为对照。在将圆盘浸入去离子水 (DW) 或乙醇:DW 后，使用高效液相色谱 (HPLC) 方法测试所有材料（商业、家庭和实验）的单体释放。使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑 (MTT) 测定法在正常人口腔成纤维细胞系 (NHOF-1) 上测试材料提取物的细胞毒性。

结果

与相应的商业产品相比，三种含有 THFM 的实验材料（F3、R3 和 R4）显示出较少或相似的单体释放。此外，与阴性对照培养基相比，两种实验材料（F3 和 F4）对 NHOF-1 细胞显示出类似的影响。

意义

一些实验性 THFM 组合物较低的单体释放和较高的细胞活力令人鼓舞。THFM 部分替代 HEMA 可能是生产生物相容性 RMGIC 的合适替代品。