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A photofabricated honeycomb micropillar array for loss-free trapping of microfluidic droplets and application to digital PCR
Lab on a Chip ( IF 6.1 ) Pub Date : 2021-08-18 , DOI: 10.1039/d1lc00629k
Yu He 1 , Zefan Lu 1 , Hongliang Fan 2 , Tao Zhang 1
Affiliation  

Droplet microfluidics is a promising platform for various biological and biomedical applications. Among which, droplet-based digital PCR (ddPCR) is one of the most challenging examples, with practical issues involving possible fusion/fission of droplets during PCR thermocycling and difficulties of indexing them for real-time monitoring. While spatially trapped droplet arrays may be helpful, they currently are either of low trapping density or suffer from high droplet loss. In this paper, we, for the first time, report a photofabricated honeycomb micropillar array (PHMA) for high-density and loss-free droplet trapping. By rationally designing high-aspect-ratio micropillars into a honeycomb configuration, droplets can be captured at a density of 160–250 droplets per mm2 and, more interestingly, without any loss. The PHMA device can be fabricated from several photocurable materials, with one gasproof photopolymer being optimally selected herein to enable the simple design to avoid sample evaporation and tedious surface modification, thereby making the fabrication very convenient. Moreover, by using a photocurable oil as a continuous phase, the trapped droplets can be further immobilized, and thus, become more stable even in PCR thermocycling. With these features, the proposed PHMA has shown promising potential in ddPCR, and is expected to find a wide range of applications in various biological and biomedical research.

中文翻译:

用于无损捕获微流体液滴的光制造蜂窝微柱阵列及其在数字 PCR 中的应用

液滴微流体是各种生物和生物医学应用的有前途的平台。其中,基于液滴的数字 PCR (ddPCR) 是最具挑战性的例子之一,其实际问题涉及 PCR 热循环过程中液滴的可能融合/裂变以及实时监测索引它们的困难。虽然空间捕获液滴阵列可能会有所帮助,但它们目前要么具有低捕获密度,要么遭受高液滴损失。在本文中,我们首次报道了一种用于高密度和无损液滴捕获的光制造蜂窝微柱阵列(PHMA)。通过合理地将高纵横比微柱设计成蜂窝状结构,可以以每毫米2 160-250 个液滴的密度捕获液滴而且,更有趣的是,没有任何损失。PHMA器件可以由多种光固化材料制成,此处优化选择一种不透气的光聚合物,使设计简单,避免样品蒸发和繁琐的表面改性,从而使制造非常方便。此外,通过使用光固化油作为连续相,可以进一步固定捕获的液滴,因此即使在 PCR 热循环中也变得更加稳定。凭借这些特点,所提出的 PHMA 在 ddPCR 中显示出巨大的潜力,有望在各种生物学和生物医学研究中找到广泛的应用。
更新日期:2021-08-27
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