The three-dimensional morphology of the Golgi apparatus in osteoclasts was investigated by computer-aided reconstruction. Rat femora were treated for nicotinamide adenine dinucleotide phosphatase (NADPase) cytochemistry, and light microscopy was used to select several osteoclasts in serial semi-thin sections to investigate the Golgi apparatus by backscattered electron-mode scanning electron microscopy. Lace-like structures with strong backscattered electron signals were observed around the nuclei. These structures, observed within the Golgi apparatus, were attributed to the reaction products (i.e., lead precipitates) of NADPase cytochemistry. Features on the images corresponding to the Golgi apparatus, nuclei, and ruffled border were manually traced and three-dimensionally reconstructed using ImageJ/Fiji (an open-source image processing package). In the reconstructed model, the Golgi apparatus formed an almost-continuous structure with a basket-like configuration, which surrounded all the nuclei and also partitioned them. This peculiar three-dimensional morphology of the Golgi apparatus was discovered for the first time in this study. On the basis of the location of the cis- and trans-sides of the Golgi apparatus and the reported results of previous studies, we postulated that the nuclear membrane synthesized specific proteins in the osteoclasts and, accordingly, the Golgi apparatus accumulated around the nuclei as a receptacle.

通过计算机辅助重建研究了破骨细胞中高尔基体的三维形态。对大鼠股骨进行烟酰胺腺嘌呤二核苷酸磷酸酶 (NADPase) 细胞化学处理，并使用光学显微镜在连续半薄切片中选择几个破骨细胞，通过背散射电子模式扫描电子显微镜研究高尔基体。在原子核周围观察到具有强烈背散射电子信号的花边状结构。在高尔基体中观察到的这些结构归因于 NADPase 细胞化学的反应产物（即铅沉淀物）。使用 ImageJ/Fiji（开源图像处理包）手动跟踪和三维重建与高尔基体、核和褶边边界对应的图像上的特征。在重建模型中，高尔基体形成了一个几乎连续的结构，呈篮状结构，包围了所有的原子核，也将它们分隔开来。在这项研究中首次发现了高尔基体的这种奇特的三维形态。根据所在位置高尔基体的顺式和反式以及先前研究报告的结果，我们假设核膜在破骨细胞中合成了特定的蛋白质，因此，高尔基体作为容器聚集在细胞核周围。