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Visualizing spatial differences in corneal electroretinogram potentials using a three-dimensional surface spline
Journal of Neural Engineering ( IF 4 ) Pub Date : 2021-09-17 , DOI: 10.1088/1741-2552/ac20e6
Brian E Kunzer 1 , Zahra Derafshi 1 , John R Hetling 1
Affiliation  

Objective. The spatial distribution of activity at the retina determines the spatial distribution of electroretinogram potentials at the cornea. Here a three-dimensional surface spline method is evaluated for interpolating corneal potentials between measurement points in multi-electrode electroretinography (meERG) data sets. Approach. 25-channel meERG responses were obtained from rat eyes before and after treatment to create local lesions. A 3rd order surface spline was used to interpolate meERG values resulting in smooth color-coded maps of corneal potentials. Potential maps were normalized using standard score values. Pre- and post-treatment responses were characterized by spatial standard deviation and by difference-from-normal plots. Main results. The spatial standard deviation for eyes with local lesions were significantly higher than for healthy eyes. The 3rd order spline resulted in well-behaved corneal potential maps that maintained low error rate when up to 30% of recording channels were excluded from analysis. Post-normalization, responses could be combined within experimental groups, and individual eyes with lesions were clearly distinguished from the healthy-eye mean response. A 3rd order surface spline is an acceptable means of interpolating meERG potentials to create corneal potential maps. The spatial standard deviation is more sensitive to local dysfunction than absolute amplitudes. Significance. This work demonstrates solutions to key challenges in the recording and analysis of meERG responses: visualization, normalization, channel loss, and identification of abnormal responses. Continued development of the meERG technique is relevant to research and clinical applications, especially where local dysfunction (early progressive disease) or local therapeutic effect (subretinal injection) is of interest.



中文翻译:

使用三维表面样条可视化角膜视网膜电图电位的空间差异

目标。视网膜活动的空间分布决定了角膜上视网膜电图电位的空间分布。这里评估了一种三维表面样条方法,用于在多电极视网膜电图 (meERG) 数据集中的测量点之间插入角膜电位。方法。在治疗前后从大鼠眼中获得 25 通道 meERG 反应以产生局部损伤。三阶表面样条用于内插 meERG 值,从而产生角膜电位的平滑彩色编码图。使用标准分值对电位图进行归一化。治疗前和治疗后的反应通过空间标准偏差和与正常图的差异来表征。主要结果. 局部病变眼的空间标准偏差显着高于健康眼。当多达 30% 的记录通道被排除在分析之外时,三阶样条导致表现良好的角膜电位图保持低错误率。标准化后,反应可以在实验组内组合,并且有病变的单个眼睛与健康眼睛的平均反应明显不同。三阶曲面样条是一种可接受的内插 meERG 电位以创建角膜电位图的方法。空间标准偏差对局部功能障碍比绝对幅度更敏感。意义. 这项工作展示了对 meERG 响应记录和分析中关键挑战的解决方案:可视化、标准化、通道丢失和异常响应的识别。meERG 技术的持续发展与研究和临床应用相关,尤其是在局部功能障碍(早期进展性疾病)或局部治疗效果(视网膜下注射)感兴趣的情况下。

更新日期:2021-09-17
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