Maintenance of mitochondrial oxidative phosphorylation capacity and other mitochondrial functions are essential for the prevention of mitochondrial dysfunction-related diseases such as neurodegenerative, cardiovascular, and liver diseases. To date, no well-known treatment modality has been developed to prevent or reduce mitochondrial dysfunction. However, a novel approach that transplants fully functional mitochondria directly into defective cells has recently caught the attention of scientists. In this review, we provide an overview of the cell/tissue source of the mitochondria to prompt cell regeneration or tissue repair in vitro and in vivo applications. The animal and human models entail that effective procedures should be used in the isolation and confirmation of mitochondrial membrane potential and function. We believe that these procedures for mitochondrial transplantation for tissue or cell culture will confirm intact, viable, and free from contamination isolated mitochondria from the appropriate sources.

中文翻译：

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成功线粒体移植的要求

维持线粒体氧化磷酸化能力和其他线粒体功能对于预防线粒体功能障碍相关疾病（如神经退行性疾病、心血管疾病和肝脏疾病）至关重要。迄今为止，尚未开发出众所周知的治疗方式来预防或减少线粒体功能障碍。然而，一种将功能齐全的线粒体直接移植到缺陷细胞中的新方法最近引起了科学家的注意。在这篇综述中，我们概述了线粒体的细胞/组织来源，以促进体外和体内细胞再生或组织修复应用程序。动物和人体模型需要使用有效的程序来分离和确认线粒体膜电位和功能。我们相信，这些用于组织或细胞培养的线粒体移植程序将确认从适当来源分离的线粒体完整、可行且无污染。