Background

Cadmium (Cd) is a representative pernicious metal, which has high biological toxicity. Its precaution through dietary administration is considered an important strategy. Considering that Portulaca oleracea L. (Por.L) has antioxidant, anti-inflammatory and other high medicinal value, and purslane insoluble dietary fiber (PIDF) has good binding property to metal ions, they could be good methods for Cd-induced biotoxicity therapy.

Purpose

To investigate the beneficial effects of Por.L or PIDF against Cd-induced subchronic toxicity and identify its underlying mechanisms.

Study design and Methods

C57BL/6 male mice (n = 12) were received 100 mg l⁻¹ CdCl₂ in water for 8 weeks. Mice were divided into four groups: Control, Cd-treated, 8% Por.L + Cd, and 8% PIDF + Cd. Histological evaluation, inductively coupled plasma-mass spectrometry, western blotting analysis, quantitative real time-PCR, gas chromatography-mass spectrometry and 16S rDNA analysis were used in the study.

Results

: Por.L treatment was able to inhibit inflammation and accumulation of Cd, enhance the activity of antioxidant enzymes, increase beneficial bacterial species of Akkermansia and Faecalibaculum and suppress the production of inflammatory cytokines in the colon, such as TNF-α, IL-6, IL-1β and IFN-γ. PIDF mainly relieved the toxicity of Cd by increasing the production of short chain fatty acids with anti-inflammatory functions and repressing the liver and kidney inflammation mediated by the TLR4/ MyD88/NF-κB pathway.

Conclusion

: Our study has demonstrated that the antagonistic-Cd effects of Por.L might be mediated via chelation, antioxidation, regulation of intestinal microecology. Thus, our study provides a novel insight into Por.L as a promising function food for the anti-Cd biotoxicity. Por.L supplement could be considered as a potential coping strategy to alleviate hazardous effects in Cd-exposed humans.

中文翻译：

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马齿苋 (Portulacae oleracea L.) 减轻镉诱导的小鼠肝肾和结肠损伤：螯合、抗氧化和肠道微生态调节的作用

背景

镉（Cd）是一种具有代表性的有害金属，具有很高的生物毒性。通过饮食进行预防被认为是一项重要的策略。考虑到马齿苋（Por.L）具有抗氧化、抗炎等高药用价值，马齿苋不溶性膳食纤维（PIDF）对金属离子具有良好的结合能力，可作为镉诱导生物毒性治疗的良好方法。 .

目的

研究 Por.L 或 PIDF 对 Cd 诱导的亚慢性毒性的有益作用并确定其潜在机制。

研究设计和方法

C57BL/6 雄性小鼠 (n = 12) 接受 100 mg l ^-1 CdCl ₂水溶液 8 周。将小鼠分为四组：对照组、Cd 处理组、8% Por.L + Cd 和 8% PIDF + Cd。研究中使用了组织学评估、电感耦合等离子体质谱法、蛋白质印迹分析、定量实时 PCR、气相色谱-质谱法和 16S rDNA 分析。

结果

： Por.L 治疗能够抑制炎症和 Cd 的积累，增强抗氧化酶的活性，增加Akkermansia和Faecalibaculum的有益细菌种类，并抑制结肠中炎症细胞因子的产生，如 TNF-α、IL-6 、IL-1β 和 IFN-γ。PIDF主要通过增加具有抗炎功能的短链脂肪酸的产生和抑制由TLR4/MyD88/NF-κB途径介导的肝肾炎症来减轻Cd的毒性。

结论

:我们的研究表明，Por.L 的拮抗镉作用可能是通过螯合、抗氧化、调节肠道微生态来介导的。因此，我们的研究为 Por.L 作为抗镉生物毒性的有前途的功能食品提供了新的见解。Por.L 补充剂可被视为一种潜在的应对策略，以减轻暴露于 Cd 的人类的有害影响。