Gastrointestinal (GI) cancers are among the most fatal diseases in the world. Numerous studies have demonstrated the relationship between autophagy and development of gastrointestinal cancers. However, whether autophagy-related genes can predict prognosis of GI cancers in individuals of Asian ancestry has not been defined. This study, evaluated the prognostic value of autophagy-related genes in gastrointestinal cancer. Expression profile of autophagy-related genes for 296 gastrointestinal cancer patients of Asian ancestry was downloaded from the TCGA database (TCGA-LIHC, TCGA-STAD, TCGA-ESCA, TCGA-PAAD, TCGA-COAD, TCGA-CHOL, and TCGA-READ). The prognostic value of the autophagy-related genes was evaluated using univariate Cox, LASSO, and multivariate Cox regression analyses. The risk score of the autophagy-related gene signature was calculated to assess its predictive prognostic value for GI cancers. Forty-seven differentially expressed autophagy-related genes, in Asian patients with gastrointestinal cancers, were identified. Of the 47 genes, 4 were associated with prognosis of GI cancer (SQSTM1, BIRC5, NRG3, and CXCR4). A prognostic model for GI cancer, based on the expression of the above 4 genes in the training set, showed that cancer patients were stratified into high-risk and low-risk groups (). The utility of the model for overall survival (OS) of GI cancer patients was consistent across the entire set, training set, and test set (entire set: ; train set: ; test set: ). The sensitivity and specificity of the ROC curve of the above prognostic model in predicting the 5-year prognosis of GI cancer was satisfactory (entire set: 0.728; train set: 0.727; test set: 0.733). Analysis of clinical samples validated the overexpression of the 4 genes (SQSTM1, BIRC5, NRG3, and CXCR4) in tumor tissues relative to paired normal tissues, consistent with bioinformatic findings. Expression of the 4 autophagy-related genes (SQSTM1, BIRC5, NRG3, and CXCR4) can accurately predict the prognosis of gastrointestinal tumors in Asian patients.

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四种自噬相关基因的表达准确预测亚洲患者胃肠癌的预后

胃肠道 (GI) 癌症是世界上最致命的疾病之一。许多研究已经证明了自噬与胃肠道癌症的发展之间的关系。然而，自噬相关基因是否可以预测亚洲血统个体胃肠道癌的预后尚未确定。本研究评估了自噬相关基因在胃肠道癌中的预后价值。从 TCGA 数据库（TCGA-LIHC、TCGA-STAD、TCGA-ESCA、TCGA-PAAD、TCGA-COAD、TCGA-CHOL 和 TCGA-READ）下载了 296 名亚洲血统胃肠癌患者的自噬相关基因的表达谱）。使用单变量 Cox、LASSO 和多变量 Cox 回归分析评估自噬相关基因的预后价值。计算自噬相关基因特征的风险评分以评估其对 GI 癌症的预测预后价值。在亚洲胃肠道癌症患者中鉴定了 47 个差异表达的自噬相关基因。在 47 个基因中，有 4 个与 GI 癌的预后相关（SQSTM1、BIRC5、NRG3 和 CXCR4）。一个基于上述 4 个基因在训练集中的表达的 GI 癌症预后模型显示，癌症患者被分为高危组和低危组。）。该模型对 GI 癌症患者总生存期 (OS) 的效用在整个数据集、训练集和测试集（整个数据集：; 动车组：; 测试集：）。上述预后模型的ROC曲线预测GI癌5年预后的敏感性和特异性均令人满意（整集：0.728；训练集：0.727；测试集：0.733）。临床样本分析验证了 4 个基因（SQSTM1、BIRC5、NRG3 和 CXCR4）在肿瘤组织中相对于成对的正常组织的过表达，这与生物信息学研究结果一致。4个自噬相关基因（SQSTM1、BIRC5、NRG3和CXCR4）的表达可以准确预测亚洲患者胃肠道肿瘤的预后。