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Microfluidic devices manufacturing with a stereolithographic printer for biological applications
Biomaterials Advances ( IF 7.9 ) Pub Date : 2021-08-26 , DOI: 10.1016/j.msec.2021.112388
Bastián Carnero 1 , Carmen Bao-Varela 1 , Ana Isabel Gómez-Varela 1 , Ezequiel Álvarez 2 , María Teresa Flores-Arias 1
Affiliation  

Stereolithographic printers have revolutionized many manufacturing processes with their capacity to easily produce highly detailed structures. In the field of microfluidics, this technique avoids the use of complex steps and equipment of the conventional technologies. The potential of low force stereolithography technology is analysed for the first time using a Form 3B printer and seven printing resins through the fabrication of microchannels and pillars. Manufacturing performance of internal and superficial channels and pillars is studied for the seven printing resins in different configurations. A complete characterization of printed structures is carried out by optical, confocal and SEM microscopy, and EDX analysis. Internal channels with unobstructed lumen are obtained for diameters and angles greater than 500 μm and 60°, respectively. Outward and inward superficial channels in the range of hundreds of microns can be fabricated with an accurate profile, printing them with a perpendicular orientation respect to the base, allowing a proper uncured resin evacuation. Outward channels are replicated by soft lithography using polydimethylsiloxane. Clear, Model and Tough resins show a good behaviour to be used as master, but Amber and Dental resins present a poor topology transference from the master to the replica. According to the needs of devices used for biological and biomedical research, transparency as well as superficial biocompatibility of some resins is evaluated. Human umbilical vein endothelial cells (HUVEC) adhesion is confirmed on Amber, Dental and Clear resins, but these cells were only able to grow and progress as a cell culture over the Amber resin. Therefore, Amber showed an adequate biocompatibility, in terms of cell adhesion and growth for HUVEC.



中文翻译:

用于生物应用的立体印刷机制造微流体装置

立体平版印刷机凭借其轻松生产高度详细结构的能力,彻底改变了许多制造工艺。在微流体领域,该技术避免了传统技术的复杂步骤和设备的使用。首次使用 Form 3B 打印机和七种打印树脂通过微通道和支柱的制造分析了低力立体光刻技术的潜力。研究了七种不同配置的印刷树脂的内部和表面通道和支柱的制造性能。印刷结构的完整表征是通过光学、共焦和 SEM 显微镜以及 EDX 分析进行的。对于直径和角度分别大于 500 μm 和 60° 的情况,获得具有通畅管腔的内部通道。数百微米范围内的向外和向内表面通道可以制造出精确的轮廓,以相对于底座的垂直方向打印它们,允许适当的未固化树脂排空。使用聚二甲基硅氧烷通过软光刻复制外向通道。Clear、Model 和 Tough 树脂在用作母版时表现出良好的性能,但 Amber 和 Dental 树脂在从母版到复制品的拓扑转移方面表现不佳。根据用于生物和生物医学研究的设备的需要,评估一些树脂的透明度和表面生物相容性。人脐静脉内皮细胞 (HUVEC) 粘附在 Amber、Dental 和 Clear 树脂上得到证实,但这些细胞只能在 Amber 树脂上作为细胞培养物生长和进展。所以,

更新日期:2021-09-03
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