ABSTRACT

This work reports the preparation of nano lignin-rich fraction material via green technology from the holistic use of lignocellulosic biomass bamboo. The bamboo is first chemically treated, followed by acid precipitation to extract bamboo-derived macro lignin-rich fraction material. The nano lignin-rich fraction material was then prepared via ultrasonication technique from the extracted bamboo-derived macro lignin-rich fraction material. The confirmation of the distinct lignin functional groups in the extracted lignin-rich fractions has been done by FTIR. Surface morphology by FESEM and TEM revealed spherical nano-lignin-rich fraction materials from extracted bamboo-derived macro lignin-rich fraction materials. DPPH assays indicated that both the obtained fractions depict beneficial antioxidant characteristics. They were found to be effective in terms of their antibacterial activity against both gram-positive bacteria Staphylococcus aureus (S.aureus) and gram-negative bacteria Escherichia coli (E.coli), using the disc diffusion method. These fractions have UV blocking property, and nano-lignin-rich fraction material acts as a more potential UV blocking agent than others. Thus, the nano-lignin-rich fraction material has great potential as a high antioxidant, antibacterial, and UV blocking agent useful in biomedical applications.

Highlights

• Extraction of macro-lignin rich fraction material using chemical treatment of lignocellulosic biomass bamboo via refluxing followed by acid precipitation.

• Preparation of nano-lignin rich fraction material from extracted bamboo-derived macro-lignin rich fraction material via ultrasonication technique as a green technology.

• Structural and surface morphology of the extracted macro-lignin & nano lignin-rich fraction materials have been analyzed by XRD, FTIR, EDX, SEM and TEM.

• The macro lignin & nano lignin-rich fraction materials showed good antioxidant, antibacterial activity and UV-blocking properties, but the nano-lignin rich fraction material exhibited more efficient properties.

摘要

这项工作报告了通过绿色技术从木质纤维素生物质竹子的整体使用中制备纳米富含木质素的馏分材料。首先对竹子进行化学处理，然后进行酸沉淀以提取源自竹子的富含大量木质素的馏分材料。然后通过超声处理技术从提取的竹子衍生的富含木质素的大颗粒材料中制备纳米富含木质素的部分材料。已通过 FTIR 确认提取的富含木质素的馏分中不同的木质素官能团。FESEM 和 TEM 的表面形态揭示了从提取的竹子衍生的富含木质素的宏观组分材料中提取的球形富含纳米木质素的组分材料。DPPH 测定表明，所获得的两种组分均具有有益的抗氧化特性。金黄色葡萄球菌 (S.aureus)和革兰氏阴性菌大肠杆菌 (E.coli)，采用纸片扩散法。这些馏分具有紫外线阻挡特性，富含纳米木质素的馏分材料比其他材料更能充当紫外线阻挡剂。因此，富含纳米木质素的组分材料具有作为高抗氧化、抗菌和紫外线阻断剂的巨大潜力，可用于生物医学应用。

强调

• 通过回流酸沉淀对木质纤维素生物质竹子进行化学处理，提取富含大量木质素的馏分材料。

• 从提取的竹子中提取的富含大量木质素的馏分材料通过超声波技术制备纳米富含木质素的馏分材料是一种绿色技术。

• 通过 XRD、FTIR、EDX、SEM 和 TEM 分析了提取的宏观木质素和富含木质素的纳米级分材料的结构和表面形态。

• 宏观木质素和纳米富含木质素部分材料显示出良好的抗氧化、抗菌活性和紫外线阻挡性能，但纳米富含木质素部分材料表现出更有效的性能。