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Characterization of Yields, Osmotic Stress-associated Traits, and Expression Patterns of ABA Receptor Genes in Winter Wheat Under Deficit Irrigation
International Journal of Plant Production ( IF 2.5 ) Pub Date : 2021-08-26 , DOI: 10.1007/s42106-021-00146-4
Xinyang Bai 1, 2 , Ruize Lin 1, 2 , Le Han 1, 2 , Kai Xiao 1, 2 , Liguo Guo 3
Affiliation  

Adoption of water-saving cultivation strategy for cereal crops benefits development of the sustainable agriculture worldwide. In this study, the effects of water supply on agronomic traits, ABA contents, drought response-associated physiological parameters, and expression patterns of the ABA receptor family genes during late stage were investigated. Under normal irrigation condition (NI, with irrigations performed prior to seed sowing, and at stages of jointing and flowering), the wheat cultivars, namely, the drought tolerant Cangmai 14 and the sensitive Jimai 325, displayed comparable agronomic traits (i.e. yields, yield components, and water use efficiencies), ABA contents, and the physiological traits associated with drought stress (i.e., proline and soluble sugar contents, and photosynthetic parameters). Under deficit irrigation (DI, without irrigation at flowering stage compared with NI), Cangmai 14 was much better on the agronomic traits, ABA contents, and the drought response-associated traits at late stage than Jimai 325. These results suggested that the improvement of drought response-associated traits contributed to the enhanced yield formation capacity of the drought-tolerant cultivars. Expression analysis on ABA receptor genes (i.e., PYL family ones) involving ABA signal perception indicated that TaPYL3, TaPYL5 and TaPYL8, three of the PYL family members, modified expression in the tested cultivars upon modified water supply copnditions, with more transcripts detected under DI than NI. Moreover, the expression levels of these genes were all shown to be higher in Cangmai 14 under DI than in Jimai 325. Transgene analysis on TaPYL3 validated that this gene exerted positive roles in modulating biomass production and photosynthetic function of plants under drought treatment. These results suggested the essential function of the ABA signaling genes in modulating plant drought response, whose enhanced transcription efficiencies positively impact on the drought response-associated physiological process, biomass production, and the yield formation capacity of wheat plants treated with DI through possibly an ABA-dependent pathway.



中文翻译:

缺水条件下冬小麦产量、渗透胁迫相关性状和 ABA 受体基因表达模式的表征

粮食作物节水栽培战略的采用有利于全球可持续农业的发展。在这项研究中,研究了供水对后期农艺性状、ABA 含量、干旱响应相关生理参数和 ABA 受体家族基因表达模式的影响。在正常灌溉条件下(NI,在播种前以及拔节和开花阶段进行灌溉),小麦品种,即耐旱的苍麦 14 和敏感的济麦 325,表现出相当的农艺性状(即产量、产量成分、水分利用效率)、ABA 含量以及与干旱胁迫相关的生理特征(即脯氨酸和可溶性糖含量以及光合参数)。亏缺灌溉 (DI, 开花期不灌水与NI相比,苍麦14在农艺性状、ABA含量、后期干旱响应相关性状等方面均优于济麦325。提高耐旱品种的产量形成能力。涉及ABA信号感知的ABA受体基因(即PYL家族基因)的表达分析表明,TaPYL3TaPYL5TaPYL8是 PYL 家族中的三个成员,在改良的供水条件下,在测试品种中的表达发生了改变,在 DI 下检测到的转录物比在 NI 下检测到的转录物多。此外,这些基因的表达水平在 DI 下苍麦 14 中均高于济麦 325。TaPYL3 的转基因分析证实该基因在调节干旱处理植物的生物量产生和光合功能方面发挥积极作用。这些结果表明 ABA 信号基因在调节植物干旱响应中的重要功能,其增强的转录效率对干旱响应相关的生理过程、生物量产生以及可能通过 ABA 进行 DI 处理的小麦植物的产量形成能力产生积极影响。 -依赖途径。

更新日期:2021-08-26
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