Bats serve as natural hosts of Pteropine orthoreovirus (PRV), an emerging group of bat-borne, zoonotic viruses. Bats appear to possess unique innate immune system responses that can inhibit viral replication, thus reducing clinical symptoms. We examined the innate immune response against PRV and assessed viral replication in cell lines derived from four bat species (Miniopterus fuliginosus, Pteropus dasymallus, Rhinolophus ferrumequinum, and Rousettus leschenaultii), one rodent (Mesocricetous auratus), and human (Homo sapiens). The expression levels of pattern recognition receptors (PRRs) (TLR3, RIG-I, and MDA5) and interferons (IFNB1 and IFNL1) were higher and PRV replication was lower in cell lines derived from M. fuliginosus, R. ferrumequinum, and R. leschenaultii. Reduction of IFNB1 expression by the knockdown of PRRs in the cell line derived from R. ferrumequinum was associated with increased PRV replication. The knockdown of RIG-I led to the most significant reduction in viral replication for all cell lines. These results suggest that RIG-I production is important for antiviral response against PRV in R. ferrumequinum.

蝙蝠是Pteropine Orthoreovirus (PRV)的天然宿主，这是一种新兴的蝙蝠传播的人畜共患病毒。蝙蝠似乎拥有独特的先天免疫系统反应，可以抑制病毒复制，从而减轻临床症状。我们检查了针对 PRV 的先天免疫反应，并评估了来自四种蝙蝠（Miniopterus fuliginosus、Pteropus dasymallus、Rhinolophus ferrumequinum和Rousettus leschenaultii）、一种啮齿动物（Mesocricetous auratus）和人类（Homo sapiens）的细胞系中的病毒复制。模式识别受体 (PRR) ( TLR3、RIG-I和MDA5 ) 和干扰素（IFNB1和IFNL1）在来自M. fuliginosus、R. ferrumequinum和R. leschenaultii的细胞系中较高，而 PRV 复制较低。通过敲除源自R. ferrumequinum的细胞系中的 PRR降低IFNB1表达与 PRV 复制增加有关。RIG-I的敲低导致所有细胞系的病毒复制最显着减少。这些结果表明，RIG-I 的产生对于R. ferrumequinum中针对 PRV 的抗病毒反应很重要。