The aim of this paper was to determine the abundance and secondary production by bacteria inhabiting the surface microlayer and subsurface water in a specific water basin, i.e., polluted estuarine harbour channel. In a 3-year seasonal cycle, the total number of bacteria and their biomass were higher in the surface microlayer (SML) 7.57 × 10⁸cells dm⁻³ and 15.86 µg C dm⁻³ than in the subsurface water (SSW) 4.25 × 10⁸cells dm⁻³ and 9.11 µg C dm⁻³ of the studied channel. The opposite relationship was noted in the level of the secondary production (SML—37.16 μg C dm⁻³ h⁻¹, SSW—60.26 μg C dm⁻³ h⁻¹) in this water basin. According to the analysed microbiological parameters, the total number of bacteria and secondary production varied along the horizontal profile in the water of the studied channel. The total number of bacteria and their secondary production showed the seasonal variation as well.

本文的目的是确定特定水域（即受污染的河口港湾）中表层微层和地下水中的细菌的丰度和二次生产。^{在 3 年的季节性周期中，表面微层 (SML) 7.57 × 10 8}个细胞 dm ^-3和 15.86 µg C dm ^-3中的细菌总数及其生物量高于地下水 (SSW) 4.25 ×所研究通道的10 ⁸个细胞 dm ^-3和 9.11 µg C dm ^-3。在二次生产水平上注意到相反的关系（SML—37.16 μg C dm ⁻³  h ⁻¹ , SSW—60.26 μg C dm ⁻³  h ⁻¹) 在这个水盆里。根据分析的微生物参数，研究渠道水中的细菌总数和二次产量沿水平剖面变化。细菌总数及其二次产量也呈现季节性变化。