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A deletion mutation within the goat AKAP13 gene is significantly associated with litter size
Animal Biotechnology ( IF 3.7 ) Pub Date : 2021-08-25 , DOI: 10.1080/10495398.2021.1968418
Xinwei Zhang 1 , Rongrong Yuan 2 , Yangyang Bai 1 , Yuta Yang 1 , Xiaoyue Song 2 , Xianyong Lan 1 , Chuanying Pan 1
Affiliation  

Abstract

A-kinase anchoring protein 13 (AKAP13) is one of the AKAP protein family members, which is correlated with estrogen receptors (ERs) and progesterone receptor (PR) activity. Consequently, the AKAP13 gene is considered to be one of the candidate genes for regulating female fertility. Hence, the objectives of this study were to discover the potential insertion/deletion (indel) variants within the AKAP13 gene and evaluate their associations with litter size of Shaanbei white cashmere goats (SBWC) to screen candidate genes for the molecular marker-assisted selection (MAS). Ultimately, we found the 16-bp deletion of AKAP13 gene which displayed three genotypes (II, ID and DD). However, it was not confirmed to Hardy-Weinberg equilibrium (HWE) in the tested population. Statistical analysis demonstrated that this 16-bp indel locus was significantly associated with litter size in goats (p < 0.05), in which the ID genotype was a key genotype for increasing litter size in goats. Besides, independent χ2 tests between different genotypes and litter size showed that high-prolific groups had higher frequency of the ‘D’ allele (p < 0.05). Briefly, AKAP13 gene is a candidate gene for improving fertility, and its 16-bp indel locus can be used as a valid DNA molecular marker for the MAS in goat breeding.



中文翻译:

山羊 AKAP13 基因内的缺失突变与产仔数显着相关

摘要

A-激酶锚定蛋白 13 (AKAP13) 是 AKAP 蛋白家族成员之一,与雌激素受体 (ER) 和孕激素受体 (PR) 活性相关。因此,AKAP13基因被认为是调控女性生育能力的候选基因之一。因此,本研究的目的是发现AKAP13基因中潜在的插入/缺失(indel)变异,并评估它们与陕北白绒山羊(SBWC)产仔数的相关性,以筛选用于分子标记辅助选择的候选基因(马航)。最终,我们发现了AKAP13的 16-bp 缺失显示三种基因型(II、ID 和 DD)的基因。然而,在测试人群中未证实哈迪-温伯格平衡(HWE)。统计分析表明,该 16-bp 插入缺失位点与山羊产仔数显着相关 ( p  < 0.05),其中 ID 基因型是增加山羊产仔数的关键基因型。此外,不同基因型和产仔数之间的独立χ 2检验表明,高产组具有更高频率的'D'等位基因( p  < 0.05)。简而言之,AKAP13基因是一个提高育性的候选基因,其16-bp的indel位点可作为山羊育种中MAS的有效DNA分子标记。

更新日期:2021-08-25
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