In this work, engineered freshwater cyanobacteria (Synechococcus elongatus PCC7942-hspA) were cultivated with CO₂ and seawater to harvest biomass, optimize biomass hydrolysis conditions to increase the sugar concentration in the hydrolysate and use it as a feedstock for the coproduction of acetoin and succinate. Since the hydrolysate contains glucose and xylose, to improve the utilization efficiency of the strain mixed sugar, Enterobacter cloacae (strain CL80) in which the budC, ldhA, and ptsG genes were knocked out was constructed. When using cyanobacteria biomass hydrolysate as the fermentation feedstock of CL80, the concentrations of acetoin and succinate were 10.55 and 1.8 g L⁻¹, respectively. This work is the first attempt to use freshwater cyanobacteria biomass hydrolysate obtained by culture with CO₂ and seawater as the feedstock for the coproduction of acetoin and succinate, and it will provide a practical strategy for reducing carbon dioxide emissions and producing biobased value-added products by the biorefinery method.

在这项工作中，工程淡水蓝藻 ( Synechococcus elongatus PCC7942-hspA) 与 CO ₂和海水一起培养以收获生物质，优化生物质水解条件以增加水解物中的糖浓度，并将其用作联产乙偶姻和琥珀酸盐的原料. 由于水解产物含有葡萄糖和木糖，为了提高菌株混合糖的利用效率，构建了其中budC、ldhA和ptsG基因被敲除的阴沟肠杆菌(菌株CL80) 。以蓝藻生物质水解物为CL80发酵原料时，乙偶姻和琥珀酸的浓度分别为10.55和1.8 g L^-1，分别。本工作首次尝试以CO ₂和海水培养获得的淡水蓝藻生物质水解物为原料联产乙偶姻和琥珀酸盐，为减少二氧化碳排放和生产生物基增值产品提供实用策略。通过生物精炼方法。