Abstract

The chronic lead intoxication was modelled by injecting outbred rats intraperitoneally with lead acetate 3 times a week for 5 weeks. Using an in vitro motility assay, it was shown that lead intoxication causes changes in in vitro actin–myosin interaction, specifically, a decrease in the maximum sliding velocity of native thin filaments over myosin isolated from the left ventricular myocardium of the rat heart. No statistically significant changes were found in the calcium sensitivity and cooperativity of the “pCa–velocity” curve, characteristics of the motile filament fraction, and isometric force. Using electrophoretic separation of proteins, a shift in the ratio of myosin heavy chains toward β-chains with a lower ATPase activity was found.

中文翻译：

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慢性铅中毒对大鼠心脏左室肌球蛋白功能特征及异构体组成的影响

摘要

慢性铅中毒模型是通过向远交大鼠腹膜内注射醋酸铅每周 3 次，共 5 周。使用体外运动试验，表明铅中毒会导致体外肌动蛋白-肌球蛋白相互作用的变化，特别是天然细丝相对于从大鼠心脏左心室心肌分离的肌球蛋白的最大滑动速度降低。在“ p Ca-速度”曲线的钙敏感性和协同性、运动细丝分数的特征和等长力方面没有发现统计学上的显着变化。使用蛋白质的电泳分离，发现肌球蛋白重链的比例向具有较低 ATP 酶活性的 β 链转移。