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Intra- and Extra-cellular Thiol–Disulfide Homeostasis in Blood of Patients with Alcohol Use Disorder
Journal of Evolutionary Biochemistry and Physiology ( IF 0.6 ) Pub Date : 2021-08-24 , DOI: 10.1134/s0022093021040207
I. Sogut 1 , A. Senat 2 , E. Kabadayi-Sahin 3 , T. Duymaz 4 , O. Erel 5
Affiliation  

Abstract

Alcohol use disorder (AUD) is the loss of a person's quality of life due to increased alcohol consumption and the failure of alcohol consumption control. In this study, the intra- and extracellular thiol-disulfide levels in people with AUD were compared with age-matched healthy controls. Forty male patients diagnosed with AUD and thirty healthy male individuals were included in the study. In addition to psychiatric and sociodemographic tests routine biochemical evaluations have been performed. The alcohol group had significantly higher Michigan Alcohol Screening Test (MAST), Beck Depression Inventory (BDI), and Beck Anxiety Inventory (BAI) scores compared to the control group. While there was a decrease in the intracellular GSH and extracellular native thiol levels in the blood of the alcohol group, GSSG and disulfide levels were increased, respectively. Subsequently, while there was an increase in the intracellular GSSG/GSH content levels in the alcohol group compared to the control group, the ratios of GSH/GSH+GSSG were decreased. These changes, indicative of oxidative stress, were observed both in blood serum and erythrocytes. Also, there was an increase in alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT) mean corpuscular volume (MCV), C-reactive protein (CRP) levels in the alcohol group in routine biochemical tests, while total protein, folic acid, ferritin and hemoglobin (HGB) levels were decreased. Our results suggest that increased intra- and extracellular GSSG/GSH % (disulfide/native thiol %) may serve as an indicator of developing oxidative stress in individuals with AUD.



中文翻译:

酒精使用障碍患者血液中的细胞内和细胞外硫醇二硫化物稳态

摘要

酒精使用障碍 (AUD) 是由于饮酒量增加和饮酒量控制失败而导致的生活质量下降。在这项研究中,将 AUD 患者的细胞内和细胞外硫醇二硫化物水平与年龄匹配的健康对照进行了比较。研究中包括了 40 名诊断为 AUD 的男性患者和 30 名健康男性。除了精神病学和社会人口学测试外,还进行了常规生化评估。与对照组相比,酒精组的密歇根酒精筛查测试 (MAST)、贝克抑郁量表 (BDI) 和贝克焦虑量表 (BAI) 得分显着更高。虽然酒精组血液中细胞内 GSH 和细胞外天然硫醇水平下降,但 GSSG 和二硫化物水平增加,分别。随后,虽然与对照组相比,酒精组的细胞内 GSSG/GSH 含量水平有所增加,但 GSH/GSH+GSSG 的比例却有所下降。在血清和红细胞中都观察到了这些表明氧化应激的变化。此外,在常规生化测试中,酒精组的丙氨酸氨基转移酶 (ALT)、天冬氨酸氨基转移酶 (AST)、γ-谷氨酰转移酶 (GGT) 平均红细胞体积 (MCV)、C 反应蛋白 (CRP) 水平增加,而总蛋白、叶酸、铁蛋白和血红蛋白 (HGB) 水平下降。我们的结果表明,细胞内和细胞外 GSSG/GSH %(二硫化物/天然硫醇 %)的增加可作为 AUD 个体发生氧化应激的指标。与对照组相比,酒精组细胞内GSSG/GSH含量水平增加,GSH/GSH+GSSG比值降低。在血清和红细胞中都观察到了这些表明氧化应激的变化。此外,在常规生化测试中,酒精组的丙氨酸氨基转移酶 (ALT)、天冬氨酸氨基转移酶 (AST)、γ-谷氨酰转移酶 (GGT) 平均红细胞体积 (MCV)、C 反应蛋白 (CRP) 水平增加,而总蛋白、叶酸、铁蛋白和血红蛋白 (HGB) 水平下降。我们的结果表明,细胞内和细胞外 GSSG/GSH %(二硫化物/天然硫醇 %)的增加可作为 AUD 个体发生氧化应激的指标。与对照组相比,酒精组细胞内GSSG/GSH含量水平增加,GSH/GSH+GSSG比值降低。在血清和红细胞中都观察到了这些表明氧化应激的变化。此外,在常规生化测试中,酒精组的丙氨酸氨基转移酶 (ALT)、天冬氨酸氨基转移酶 (AST)、γ-谷氨酰转移酶 (GGT) 平均红细胞体积 (MCV)、C 反应蛋白 (CRP) 水平增加,而总蛋白、叶酸、铁蛋白和血红蛋白 (HGB) 水平下降。我们的结果表明,细胞内和细胞外 GSSG/GSH %(二硫化物/天然硫醇 %)的增加可作为 AUD 个体发生氧化应激的指标。

更新日期:2021-08-25
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