Abstract

For the past few years, organic-inorganic hybrid nanocarriers have been widely explored for effective drug delivery and preferable disease treatments. In this article, hydrothermal method was utilized to prepare fine dispersed layered double hydroxide (Mg-Al LDH) suspension. Polyethylene glycol (PEG) was grafted on the surface of LDH lamella in order to improve the dispersibility of LDH. Besides, the anti-cancer drug gemcitabine was grafted on the surface of LDH lamellas through chemical grafting. Hence a novel new type of organic-inorganic hybrid drug delivery system LDH-mPEG-Gemcitabine was obtained. In addition, the siRNA was intercalated into the LDH interlamination by ion exchange method to realize drug and gene co-delivery. The loading capacity of LDH and LDH-mPEG-Gemcitabine was evaluated by agarose gel electrophoresis. The characterization by laser particle size analyzer, TEM, FT-IR, XRD, in vitro cell viability and in vitro drug release demonstrated that LDH-mPEG-Gemcitabine possessed fine dispersibility, uniform morphology and particle size, fine biocompatibility, ideal drug loading and releasing capacity and held great potential to be used as a desired co-delivery system for drug and gene.

摘要

在过去的几年中，有机-无机杂化纳米载体已被广泛探索用于有效的药物递送和优选的疾病治疗。本文采用水热法制备了细分散的层状双氢氧化物（Mg-Al LDH）悬浮液。为了提高LDH的分散性，在LDH薄片表面接枝了聚乙二醇(PEG)。此外，通过化学接枝将抗癌药物吉西他滨接枝在LDH薄片表面。由此获得了一种新型的有机-无机杂化给药系统LDH-mPEG-吉西他滨。此外，通过离子交换法将siRNA嵌入LDH层间，实现药物和基因的共递送。LDH 和 LDH-mPEG-吉西他滨的负载能力通过琼脂糖凝胶电泳进行评估。体外细胞活力和体外药物释放表明，LDH-mPEG-吉西他滨具有良好的分散性、均匀的形态和粒径、良好的生物相容性、理想的载药和释药能力，具有作为理想共递送系统的巨大潜力。药物和基因。