A simple and effective method was established and validated to determine 4-methylumbelliferone (4-MU) for screening the natural neuraminidase inhibitors (NAIs) from traditional medicines (TMs) by high performance liquid chromatography combined with fluorescence detection (HPLC-FLD). 4-MU and TMs compounds were separated on a Hedera TM ODS column (5 μm, 4.6 × 250 mm) using an isocratic elution of 55% methanol at 35°C. The flow rate was 1 mL min⁻¹. The excitation and emission wavelength were performed at 320 nm and 480 nm. Some extracts of TMs and compounds were selected as examples to demonstrate the feasibility of the new HPLC-FLD method. It was found that the results of most compounds except for the auto fluorescence substances determined by HPLC-FLD were in good agreement with NA enzyme-based inhibitory assays. Comparing to traditional NA enzyme-based inhibitory assays, the HPLC-FLD method could prevent interference from fluorescence pigments of compounds. It was considered a simple, effective, and economical technique for the screening the natural neuraminidase inhibitors from traditional medicines.

中文翻译：

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HPLC-FLD 评价中药复方和提取物的神经氨酸酶抑制活性

建立并验证了一种简单有效的方法来测定 4-甲基伞形酮 (4-MU)，用于通过高效液相色谱结合荧光检测 (HPLC-FLD) 筛选传统药物 (TM) 中的天然神经氨酸酶抑制剂 (NAI)。4-MU和TMS化合物上分离常春藤TM ODS柱（5  μ使用在35℃下55％甲醇的等度洗脱米，4.6×250毫米）。流速为 1 mL min ^-1. 激发和发射波长在 320 nm 和 480 nm 处进行。选择了一些 TM 和化合物的提取物作为示例，以证明新的 HPLC-FLD 方法的可行性。结果表明，除自发荧光物质外，大多数化合物的结果与 NA 酶抑制法测定的结果非常吻合。与传统的基于 NA 酶的抑制测定相比，HPLC-FLD 方法可以防止化合物荧光色素的干扰。它被认为是一种从传统药物中筛选天然神经氨酸酶抑制剂的简单、有效和经济的技术。