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Inhibition of hypoxia-inducible factor-1 by salidroside in an in vitro model of choroidal neovascularization
Cutaneous and Ocular Toxicology ( IF 1.6 ) Pub Date : 2022-07-15 , DOI: 10.1080/15569527.2021.1973023
Haitao Yang 1 , Qingwu Yang 1 , Linfei Zheng 1
Affiliation  

Abstract

Purpose

As a characteristic of age-related macular degeneration (AMD), choroidal neovascularization (CNV) causes severe vision loss. The current treatment has limited efficacy. This study was to investigate effects of Salidroside against CNV and explore its underlying mechanisms.

Methods

RF/6A cells were treated with 200 mM cobalt chloride (CoCl2) for 6 hr to mimic hypoxic condition. Cells were then treated with Salidroside at 10, 30, and 100 µM for 24 hr. Cells treated with DMSO were used as negative control. The cell proliferation was assessed using 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium-bromid assay. The tube formation was investigated on Matrigel. The cell migration was measured by a Transwell assay. RT-qPCR was used to detect the gene expression. Immuohistochemistry and western blot were used to detect the expression of proteins.

Results

Salidroside significantly inhibited the cell migration and tube formation activity of RF/6A cells under hypoxia. Moreover, Salidroside reduced the expression levels of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 (HIF-1) in RF/6A cells.

Conclusions

Our data suggested that Salidroside could be a potential novel therapeutic agent against CNV.



中文翻译:

红景天苷对脉络膜新生血管体外模型中缺氧诱导因子1的抑制作用

摘要

目的

作为年龄相关性黄斑变性 (AMD) 的一个特征,脉络膜新生血管 (CNV) 会导致严重的视力丧失。目前的治疗效果有限。本研究旨在研究红景天苷对 CNV 的作用并探讨其潜在机制。

方法

RF/6A 细胞用 200 mM 氯化钴 (CoCl 2 ) 处理 6 小时以模拟缺氧条件。然后用 10、30 和 100 µM 的红景天苷处理细胞 24 小时。用 DMSO 处理的细胞用作阴性对照。使用 3-(4,5-二甲基噻唑)-2,5-二苯基四唑溴化物测定法评估细胞增殖。在 Matrigel 上研究了管的形成。通过 Transwell 测定法测量细胞迁移。RT-qPCR用于检测基因表达。免疫组织化学和蛋白质印迹用于检测蛋白质的表达。

结果

红景天苷显着抑制缺氧条件下RF/6A细胞的细胞迁移和成管活性。此外,红景天苷降低了 RF/6A 细胞中血管内皮生长因子 (VEGF) 和缺氧诱导因子-1 (HIF-1) 的表达水平。

结论

我们的数据表明,红景天苷可能是一种潜在的新型 CNV 治疗剂。

更新日期:2022-07-15
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