Abstract

Extracellular proteinase of the opportunistic Aspergillus fumigatus D-1 strain (molecular weight ~33 kDa, pI 4.6) was isolated. It was shown that proteinase hydrolyzes casein, fibrin, fibrinogen, albumin, and hemoglobin to varying degrees. However, proteolytic activity with respect to globular proteins of blood plasma was comparable to fibrinolytic activity. Proteinase did not coagulate human fibrinogen and bovine fibrinogen; it also did not coagulate human and rabbit blood plasma without dilution and when diluted twice. The plasminogen-activating activity of A. fumigatus D-1 extracellular proteinase was found, which may indicate its ability to indirect fibrinolysis.

中文翻译：

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烟曲霉蛋白酶对血浆蛋白的影响

摘要

分离出机会性烟曲霉D-1 菌株的胞外蛋白酶（分子量 ~33 kDa，pI 4.6）。结果表明，蛋白酶在不同程度上水解酪蛋白、纤维蛋白、纤维蛋白原、白蛋白和血红蛋白。然而，血浆球状蛋白的蛋白水解活性与纤溶活性相当。蛋白酶不凝固人纤维蛋白原和牛纤维蛋白原；它也不会在未经稀释和稀释两次时使人和兔血浆凝固。发现烟曲霉D-1胞外蛋白酶的纤溶酶原激活活性，这可能表明其具有间接纤溶的能力。