Objectives

Proteases have gained great attention due to their enormous applications in food, tannery, detergent, photography and many other industries. Proteases rank third position in the production of enzymes. This paper targets to isolate a bacterium with high alkaline protease activity and optimization of its production conditions using Response Surface Methodology (RSM).

Results

A bacterium isolated from soil contaminated with detergent exhibited clearance zone on skim milk agar medium with a protease activity of 22 U/ml. The bacterial strain was identified as Bacillus cereus KM05 and optimization of its production conditions were performed using statistical methods. Further optimization with Box Behnken design resulted in an increase in protease activity by 1.5-fold (28.6 U/ml). The protease enzyme was thermotolerant up to 70 °C with stability towards alkaline pH (pH 9). The enzyme was not affected by most of the metal ions and solvents. Moreover, the protease was also compatible with six commercial detergents tested. Densitometric analysis of the destained fabric materials following the detergent-enzyme treatment, revealed a stain removal efficiency of 97%.

Conclusion

The alkaline protease enzyme obtained was stable at different conditions with stain removal efficacy. Hence, the present alkaline protease could be used for detergent formulations.

中文翻译：

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使用响应面法对蜡样芽孢杆菌 KM 05 的热稳定性碱性蛋白酶进行统计优化

目标

蛋白酶因其在食品、制革、洗涤剂、摄影和许多其他行业中的巨大应用而受到极大关注。蛋白酶在酶的生产中排名第三。本文旨在分离一种具有高碱性蛋白酶活性的细菌，并使用响应面方法 (RSM) 优化其生产条件。

结果

从被洗涤剂污染的土壤中分离出的细菌在蛋白酶活性为 22 U/ml 的脱脂牛奶琼脂培养基上显示出清除区。该菌株被鉴定为蜡样芽孢杆菌KM05，并使用统计方法对其生产条件进行优化。Box Behnken 设计的进一步优化导致蛋白酶活性增加 1.5 倍 (28.6 U/ml)。该蛋白酶在高达 70 °C 的温度下具有耐热性，并且对碱性 pH (pH 9) 具有稳定性。该酶不受大多数​​金属离子和溶剂的影响。此外，蛋白酶还与测试的六种商业洗涤剂相容。洗涤剂酶处理后脱色织物材料的光密度分析表明，去污效率为 97%。

结论

所得碱性蛋白酶在不同条件下稳定，具有去污功效。因此，本碱性蛋白酶可用于洗涤剂制剂。