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Effects of VO2 nanoparticles on human liver HepG2 cells: Cytotoxicity, genotoxicity, and glucose and lipid metabolism disorders
NanoImpact ( IF 4.9 ) Pub Date : 2021-08-25 , DOI: 10.1016/j.impact.2021.100351
Jia-Bei Li 1 , Wen-Song Xi 1 , Shi-Ying Tan 1 , Yuan-Yuan Liu 1 , Hao Wu 1 , Yuanfang Liu 2 , Aoneng Cao 1 , Haifang Wang 1
Affiliation  

The rapid development of smart materials stimulates the production of vanadium dioxide (VO2) nanomaterials. This significantly increases the population exposure to VO2 nanomaterials via different pathways, and thus urges us to pay more attentions to their biosafety. Liver is the primary accumulation organ of nanomaterials in vivo, but the knowledge of effects of VO2 nanomaterials on the liver is extremely lacking. In this work, we comprehensively evaluated the effects of a commercial VO2 nanoparticle, S-VO2, in a liver cell line HepG2 to illuminate the potential hepatic toxicity of VO2 nanomaterials. The results indicated that S-VO2 was cytotoxic and genotoxic to HepG2 cells, mainly by inhibiting the cell proliferation. Apoptosis was observed at higher dose of S-VO2, while DNA damage was detected at all tested concentrations. S-VO2 particles were internalized by HepG2 cells and kept almost intact inside cells. Both the particle and dissolved species of S-VO2 contributed to the observed toxicities. They induced the overproduction of ROS, and then caused the mitochondrial dysfunction, ATP synthesis interruption, and DNA damages, consequently arrested the cell cycle in G2/M phase and inhibited the proliferation of HepG2 cells. The S-VO2 exposure also resulted in the upregulations of glucose uptake and lipid content in HepG2 cells, which were attributed to the ROS production and autophagy flux block, respectively. Our findings offer valuable insights into the liver toxicity of VO2 nanomaterials, benefiting their safely practical applications



中文翻译:

VO2 纳米颗粒对人肝 HepG2 细胞的影响:细胞毒性、遗传毒性和糖脂代谢紊乱

智能材料的快速发展刺激了二氧化钒(VO 2)纳米材料的生产。这显着增加了通过不同途径接触VO 2纳米材料的人群,从而促使我们更加关注它们的生物安全性。肝脏是纳米材料在体内的主要聚集器官,但关于VO 2纳米材料对肝脏的影响的知识却极为缺乏。在这项工作中,我们全面评估了商业 VO 2纳米颗粒 S-VO 2在肝细胞系 HepG2 中的作用,以阐明 VO 2纳米材料的潜在肝毒性。结果表明,S-VO 2对HepG2细胞具有细胞毒性和遗传毒性,主要通过抑制细胞增殖。在更高剂量的 S-VO 2下观察到细胞凋亡,而在所有测试浓度下都检测到 DNA 损伤。S-VO 2颗粒被 HepG2 细胞内化并在细胞内保持几乎完整。S-VO 2的颗粒和溶解的物质都促成了观察到的毒性。它们诱导ROS过度产生,进而引起线粒体功能障碍、ATP合成中断和DNA损伤,从而使细胞周期停滞在G2/M期,抑制HepG2细胞的增殖。S-VO 2暴露还导致 HepG2 细胞中葡萄糖摄取和脂质含量的上调,这分别归因于 ROS 的产生和自噬通量阻断。我们的研究结果为VO 2纳米材料的肝毒性提供了有价值的见解,有利于它们的安全实际应用

更新日期:2021-08-30
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