当前位置: X-MOL 学术Biosens. Bioelectron. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Efficient and robust dual modes of fluorescence sensing and smartphone readout for the detection of pyrethroids using artificial receptors bound inside a covalent organic framework
Biosensors and Bioelectronics ( IF 12.6 ) Pub Date : 2021-08-25 , DOI: 10.1016/j.bios.2021.113582
Wei Jiang 1 , Yuan Zhao 1 , Dianwei Zhang 1 , Xuecheng Zhu 1 , Huilin Liu 1 , Baoguo Sun 1
Affiliation  

Rapid, specific, sensitive, instrument-free, and visual sensors are useful for a variety of applications for on-site detection. In the study, Wwe have developed an efficient and robust method using dual modes of fluorescence sensing and smartphone readout for the detection of pyrethroids using artificial receptors inside a covalent organic framework. Carbazole-conjugated frameworks (CCFs) were used to prepare efficient fluorescent probes that combine stability with light-emitting activity. C=N linkages between aldehydes and amines formed Schiff bases, allowing the development of layered structures, creating exceptionally stable frameworks. Artificial receptors that can bind compounds inside the CCFs with high affinity, for both the detection and absorption of λ-cyhalothrin (LC), were constructed using room-temperature reverse microemulsion polymerization. Under optimum conditions, the fluorescence sensing correlation with the concentration of LC showed good linearity in the range of 0.8-175 μg L−1 with a detection limit of 0.368 μg L−1. The smartphone-based visible readout exhibited a good effect, with a detection limit of 4.067 μg L-1, and recovery of 887.64 %-103.45 % in food samples. A parallel analysis in food samples was conducted by high-performance liquid chromatography, the results showed good consistency, indicating the practicability of the developed method. Dual mode analysis can avoid the disadvantages of a single response, providing excellent sensitivity, specificity, and efficiency through a strong binding force between the target and the artificial receptors.



中文翻译:

使用结合在共价有机框架内的人工受体检测拟除虫菊酯的高效和稳健的荧光传感和智能手机读出双重模式

快速、特异性、灵敏、无需仪器和视觉传感器可用于各种现场检测应用。 在研究中, 我们开发了一种使用荧光传感和智能手机读出双重模式的有效且稳健的方法,用于使用共价有机框架内的人工受体检测拟除虫菊酯。咔唑共轭框架 (CCF) 用于制备结合稳定性和发光活性的高效荧光探针。醛和胺之间的 C=N 键形成席夫碱,允许分层结构的发展,创造异常稳定的框架。人工受体可以以高亲和力结合 CCF 内的化合物,用于检测和吸收 λ-三氯氟氰菊酯 (LC),使用室温反相微乳液聚合构建。在最佳条件下,荧光传感与LC浓度的相关性在0范围内表现出良好的线性。-1的检测限为 0.368 μg L -1。基于智能手机的可见光读数显示出良好的效果,检测限为4.067 μg L -1,回收率为887.64 %-103.45% 在食品样品中。采用高效液相色谱法对食品样品进行平行分析,结果一致性好,表明所建立方法的实用性。双模式分析可以避免单一响应的缺点,通过靶标与人工受体之间的强结合力提供出色的灵敏度、特异性和效率。

更新日期:2021-08-25
down
wechat
bug